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      In vitro Accumulation of 14C-Xanthine by Rabbit Renal Cortex and its Relationship to Overall Oxypurine Transport

      Nephron

      S. Karger AG

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          Abstract

          Previous detailed studies of the transport of hypoxanthine and uric acid indicate that these two oxypurines are handled by rather different processes. This report gives information on another important oxypurine, xanthine. Rabbit renal cortex slices were found to accumulate <sup>14</sup>C-xanthine to concentrations significantly above those in the bathing solution (slice: medium or S/M ratios of about 2.0). This uptake is energy dependent as indicated by the effects of metabolic inhibitors. Carrier mediation was also probably involved and a relatively sharp pH optimum was noted. A requirement for potassium was also observed, but it was not nearly as marked as that seen for uric acid. Various agents (proben-ecid, octanoate, decanoate, etc.) known to inhibit organic acid transport were tested and found to reduce xanthine uptake significantly. The non-metabolizable amino acid, α-aminoisobutyric acid, did not block accumulation, however. Several organic bases were also without effect. A most significant finding was that several purine substances (including adenine and guanine) did not block accumulation. In general these data indicate that xanthine is transported mainly by the organic acid transport system (i.e. p-aminohippurate system) and not by the processes that handle hypoxanthine.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1970
          1970
          26 November 2008
          : 7
          : 4
          : 339-349
          Affiliations
          Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH
          Article
          179834 Nephron 1970;7:339–349
          10.1159/000179834
          5426044
          © 1970 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 11
          Categories
          Paper

          Cardiovascular Medicine, Nephrology

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