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      The Stability of pH, P CO 2 , and Calculated [HCO 3] of Urine Samples Collected under Oil

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          Abstract

          In order to determine the stability of directly measured pH and Pco<sub>2</sub>, and calculated [HCO<sub>3</sub>] in stored urine, 11 alkali-loaded normal subjects provided 33 spot and sixteen 24-hour mineral oil-covered, thymol-preserved, refrigerated urine samples. For the spot samples, pH and Pco<sub>2</sub> were measured immediately and again at 4 and 24 h. In addition, immediately after voiding, 24 of the spot samples were split into oil-covered and no-oil moieties and analyzed immediately and again at 4 and 24 h. pH and Pco<sub>2</sub> measurements of the 24-hour collections were carried out immediately after completion and again 24 h later. The results demonstrated the importance of using oil to limit the escape of CO<sub>2</sub> from stored urine. Thus, after 24 h the oil-uncovered subgroup of 24 spot urine samples sustained a 82% decline in Pco<sub>2</sub> and a 20% fall in [HCO<sub>3</sub>]. In contrast, the corresponding percentage decrements in the oil-covered samples were 16 and 1%, respectively. The results also indicated that even with oil there is loss of CO<sub>2</sub>, which increases with time and which shows a statistically significant direct correlation with the baseline level of Pco<sub>2</sub>· Nevertheless, modest loss of CO<sub>2</sub> usually produces only slight decrements in [HCO<sub>3</sub>] because of the countervailing influence of the resultant increase in pH. We conclude that the use of mineral oil is necessary in order to obtain adequate stability of Pco<sub>2</sub>, pH and [HCO<sub>3</sub>] in refrigerated urine requiring several hours or more of storage.

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          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1988
          1988
          09 December 2008
          : 50
          : 4
          : 320-324
          Affiliations
          Medical and Research Services, Veterans Administration Medical Center, and Department of Medicine, University of Miami, School of Medicine, Miami, Fla., USA
          Article
          185196 Nephron 1988;50:320–324
          10.1159/000185196
          3148868
          © 1988 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 5
          Categories
          Original Paper

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