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      Determination of the Content of Water in Bovine Corneas by Differential Scanning Calorimetry and Thermogravimetric Analysis


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          The ‘bound’, ‘bulk’, and total water contents in bovine corneas were investigated using differential scanning calorimetry and thermogravimetric analysis. The amount of total water (expressed as a function of total wet mass) was observed to be 83.7 ± 1.1 % using lyophilization and 84.1 ± 0.8% using thermogravimetric analysis. These values are not significantly different from one another, but some discrepancies are observed when these values are compared with the values reported in the literature, thus indicating a need for guidelines concerning sample handling and analysis of these tissues. The ‘bound’ water was observed to be 62.3 ± 2.7 (% of total water) and the amount of’bulk’ water was calculated to be 20.8 ± 2.5 (% of total water).

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 26
          : 2
          : 105-109
          Department of Chemistry, The College of Wooster, Ohio, USA
          267399 Ophthalmic Res 1994;26:105–109
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 29 July 1993
          : 28 September 1993
          Page count
          Pages: 5
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Differential scanning calorimetry,Thermogravimetric analysis,Water,Cornea,Bovine eye


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