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      Functional extension of high-speed AFM for wider biological applications.

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          Abstract

          High-speed atomic force microscopy (HS-AFM) has been established and used, which can visualize biomolecules in dynamic action at high spatiotemporal resolution without disturbing their function. Various studies conducted in the past few years have demonstrated that the dynamic structure and action of biomolecules revealed with HS-AFM can provide greater insights than ever before into how the molecules function. However, this microscopy has still limitations in some regards. Recently, efforts have been carried out to overcome some of the limitations. As a result, it has now become possible to visualize dynamic processes occurring even on live cells and perform simultaneous observations of topographic and fluorescent images at a high rate. In this review, we focus on technical developments for expanding the range of objects and phenomena observable by HS-AFM as well as for granting multiple functionalities to HS-AFM.

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          Author and article information

          Journal
          Ultramicroscopy
          Ultramicroscopy
          Elsevier BV
          1879-2723
          0304-3991
          Jan 2016
          : 160
          Affiliations
          [1 ] Department of Physics, Kanazawa University, Kanazawa 920-1192, Japan; Bio-AFM Frontier Research Center, Kanazawa University, Kanazawa 920-1192, Japan; CREST/JST, Tokyo 102-0075, Japan.
          [2 ] Department of Physics, Kanazawa University, Kanazawa 920-1192, Japan.
          [3 ] Department of Physics, Kanazawa University, Kanazawa 920-1192, Japan; Bio-AFM Frontier Research Center, Kanazawa University, Kanazawa 920-1192, Japan; CREST/JST, Tokyo 102-0075, Japan. Electronic address: tando@staff.kanazawa-u.ac.jp.
          Article
          S0304-3991(15)30050-4
          10.1016/j.ultramic.2015.10.017
          26521164
          8a1b9b10-8058-4861-91c5-d2437a757127
          History

          Imaging,AFM,Biomolecules,High-speed AFM,Live cells
          Imaging, AFM, Biomolecules, High-speed AFM, Live cells

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