Sete Cidades National Park is located in northeastern Piauí State, Brazil, and comprises several vegetation types characteristic of the Brazilian Cerrado. This study contributes to understanding the composition and distribution of Brazilian bryoflora and provides a list of bryophytes of Sete Cidades National Park. A total of 520 samples representing 90 species were collected, of which 53 represent new occurrences for Piauí, 25 for the Cerrado and four for the Northeast Region of Brazil. About 55% of the species have broad distributions in the country. This study highlights the relevance of Sete Cidades National Park for the conservation of species and the importance of continued bryofloristic studies in Piauí.