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      Proteomic analysis of elicitation of downy mildew disease resistance in pearl millet by seed priming with β-aminobutyric acid and Pseudomonas fluorescens.

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          Abstract

          Downy mildew is one of the severe diseases of pearl millet, globally affecting its commercial production. Priming of seeds of a susceptible cultivar of pearl millet with β-aminobutyric acid (BABA) and Pseudomonas fluorescens has reduced the downy mildew disease incidence level under field studies. In the current study, proteomic approach was used to elucidate the poorly studied resistance mechanism in these elicitor primed pearl millet seeds in response to Sclerospora graminicola infection. 2DE-MS/MS based proteomic approach revealed that majority of the 63 differentially accumulated (p≤0.05) proteins associated with energy and metabolism followed by stress and defense category. Multivariate statistics disclosed that infection caused by the pathogen rather than elicitor treatment had a major influence on the dynamics of protein abundance. Mechanism of priming mediated by BABA and P. fluorescens were different from each other as evident by the protein abundance profile of hierarchical clustering analysis. Over-representation of proteins pertaining to glucose metabolism suggests that seed priming ensures plant protection against disease without compromising its normal growth and development. In addition the study forms a basis for future investigation by functional analysis of these differentially accumulated proteins to further unravel the resistance mechanism of elicitor primed plant against the S. graminicola.

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          Author and article information

          Journal
          J Proteomics
          Journal of proteomics
          Elsevier BV
          1876-7737
          1874-3919
          Apr 29 2015
          : 120
          Affiliations
          [1 ] Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysore 570 006, Karnataka, India.
          [2 ] HR-LCMS Lab Sophisticated Analytical Instrumentation Facility, Indian Institute of Technology, Bombay, India.
          [3 ] Camo Software, Bangalore, India.
          [4 ] Department of Studies in Biotechnology, Manasagangotri, University of Mysore, Mysore 570 006, Karnataka, India. Electronic address: krk@appbot.uni-mysore.ac.in.
          Article
          S1874-3919(15)00077-9
          10.1016/j.jprot.2015.02.013
          25746381

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