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      Secreted factors from cultured dental pulp stem cells promoted neurite outgrowth of dorsal root ganglion neurons and ameliorated neural functions in streptozotocin‐induced diabetic mice

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          Abstract

          Aims/Introduction

          Transplantation of stem cells promotes axonal regeneration and angiogenesis in a paracrine manner. In the present study, we examined whether the secreted factors in conditioned medium of stem cells from human exfoliated deciduous teeth ( SHEDCM) had beneficial effects on diabetic polyneuropathy in mice.

          Materials and Methods

          Conditioned medium of stem cells from human exfoliated deciduous teeth was collected 48 h after culturing in serum‐free Dulbecco's modified Eagle's medium ( DMEM), and separated into four fractions according to molecular weight. Dorsal root ganglion neurons from C57 BL/6J mice were cultured with SHEDCM or DMEM to evaluate the effect on neurite outgrowth. Streptozotocin‐induced diabetic mice were injected with 100 μL of SHEDCM or DMEM into the unilateral hindlimb muscles twice a week over a period of 4 weeks. Peripheral nerve functions were evaluated by the plantar test, and motor and sensory nerve conduction velocities. Intraepidermal nerve fiber densities, capillary number‐to‐muscle fiber ratio, capillary blood flow and morphometry of sural nerves were also evaluated.

          Results

          Conditioned medium of stem cells from human exfoliated deciduous teeth significantly promoted neurite outgrowth of dorsal root ganglion neurons compared with DMEM. Among four fractions of SHEDCM, the only fraction of <6  kDa promoted the neurite outgrowth of dorsal root ganglion neurons. In addition, SHEDCM significantly prevented decline in sensory nerve conduction velocities compared with DMEM in diabetic mice. Although SHEDCM did not improve intraepidermal nerve fiber densities or morphometry of sural nerves, SHEDCM ameliorated the capillary number‐to‐muscle fiber ratio and capillary blood flow.

          Conclusions

          These results suggested that SHEDCM might have a therapeutic effect on diabetic polyneuropathy through promoting neurite outgrowth, and the increase in capillaries might contribute to the improvement of neural function.

          Abstract

          In this study, we examined whether the secreted factors could be collected from conditioned medium of stem cells from human exfoliated deciduous teeth had beneficial effects on diabetic polyneuropathy. Conditioned medium of stem cells from human exfoliated deciduous teeth might have a therapeutic effect on diabetic polyneuropathy through promoting neurite outgrowth, and the increase in capillaries might contribute to the improvement of neural function.

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          Most cited references31

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          Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine.

          To date, 5 different human dental stem/progenitor cells have been isolated and characterized: dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from apical papilla (SCAP), and dental follicle progenitor cells (DFPCs). These postnatal populations have mesenchymal-stem-cell-like (MSC) qualities, including the capacity for self-renewal and multilineage differentiation potential. MSCs derived from bone marrow (BMMSCs) are capable of giving rise to various lineages of cells, such as osteogenic, chondrogenic, adipogenic, myogenic, and neurogenic cells. The dental-tissue-derived stem cells are isolated from specialized tissue with potent capacities to differentiate into odontogenic cells. However, they also have the ability to give rise to other cell lineages similar to, but different in potency from, that of BMMSCs. This article will review the isolation and characterization of the properties of different dental MSC-like populations in comparison with those of other MSCs, such as BMMSCs. Important issues in stem cell biology, such as stem cell niche, homing, and immunoregulation, will also be discussed.
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            The biology of vascular endothelial growth factor.

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              • Record: found
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              • Article: not found

              Diabetic neuropathies: a statement by the American Diabetes Association.

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                Author and article information

                Contributors
                hkamiya@aichi-med-u.ac.jp
                Journal
                J Diabetes Investig
                J Diabetes Investig
                10.1111/(ISSN)2040-1124
                JDI
                Journal of Diabetes Investigation
                John Wiley and Sons Inc. (Hoboken )
                2040-1116
                2040-1124
                21 June 2019
                January 2020
                : 11
                : 1 ( doiID: 10.1111/jdi.v11.1 )
                : 28-38
                Affiliations
                [ 1 ] Division of Diabetes Department of Internal Medicine Aichi Medical University School of Medicine Nagakute Japan
                [ 2 ] Department of Internal Medicine School of Dentistry Aichi Gakuin University Nagoya Japan
                [ 3 ] Department of Oral and Maxillofacial Surgery Nagoya University Graduate School of Medicine Nagoya Japan
                [ 4 ] Department of Histology and Oral Histology Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan
                Author notes
                [*] [* ] Correspondence

                Hideki Kamiya

                Tel.: +81‐561‐62‐1683

                Fax: +81‐561‐62‐1276

                E‐mail address: hkamiya@ 123456aichi-med-u.ac.jp

                Author information
                https://orcid.org/0000-0002-5477-5886
                https://orcid.org/0000-0002-0062-0694
                Article
                JDI13085
                10.1111/jdi.13085
                6944849
                31144464
                8a4203f2-38ba-4431-876e-e756d184c285
                © 2019 The Authors Journal of Diabetes Investigation published by Asian Association for the Study of Diabetes (AASD) and John Wiley & Sons Australia, Ltd

                This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

                History
                : 21 December 2018
                : 18 May 2019
                : 21 May 2019
                Page count
                Figures: 4, Tables: 2, Pages: 11, Words: 6742
                Funding
                Funded by: Grant‐in‐Aid for Scientific Research
                Award ID: 15H06720
                Award ID: 16K09742
                Award ID: 17K09851
                Funded by: Japan Diabetes Foundation
                Funded by: Manpei Suzuki Diabetes Foundation , open-funder-registry 10.13039/501100007664;
                Funded by: Aichi Medical University Aikeikai
                Funded by: Japan Association for Diabetes Education and Care
                Categories
                Original Article
                Articles
                Basic Science and Research
                Custom metadata
                2.0
                January 2020
                Converter:WILEY_ML3GV2_TO_JATSPMC version:5.7.4 mode:remove_FC converted:07.01.2020

                conditioned medium,diabetic polyneuropathy,stem cells from human exfoliated deciduous teeth

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