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      Dual Immunodetection and Hybridisation in situ of Opsin mRNA and Rhodopsin Protein in Retinal Sections

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          We describe a simple method for combining in situ hybridisation and immunohistochemistry on the same retinal section. The technique was developed using a radiolabelled cDNA probe for opsin and an antibody (ROS1F4) against rhodopsin. This method retains the antigenic sites if immunocytochemistry is performed prior to in situ hybridisation. Opsin mRNA was found in the photoreceptor inner segment with rhodopsin immunolocalised to the photoreceptor outer segments. The technique should be applicable to numerous situations including analysis of the sequence of events in the expression and synthesis of the various opsins during retinal development and degeneration.

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          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          11 December 2009
          : 28
          : 5
          : 296-302
          aUniversity Department of Ophthalmology, Manchester Royal Eye Hospital, Manchester, bBritish Retinitis Pigmentosa Society Laboratory, Department of Pharmacology, The Rayne Institute, UMDS, London, and cDepartment of Pathology, University of Cambridge, UK
          267918 Ophthalmic Res 1996;28:296–302
          © 1996 S. Karger AG, Basel

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          Pages: 7
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