There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
This study was conducted to evaluate whether the sex of donor primordial germ cells
(PGCs) influences production of chimeric semen from recipient hatchlings produced
by interspecies transfer between pheasant (Phasianus colchicus) and chicken (Gallus
gallus). Pheasant PGCs were retrieved from 7-d-old embryos and subsequently transferred
into circulatory blood of 2.5-d-old (Stage 17) embryos. The sex of embryos was discerned
3 to 6 days after laying, and in preliminary study, overall rate of embryo survival
after sexing was 74.6% with male-to-female ratio of 0.49 to 0.51. In Experiment 1,
magnetic-activated cell sorting (MACS) using QCR1 antibody was effective for enriching
the population of male and female PGCs in gonadal cells (9.2- to 12.5-fold and 10.8-
to 19.5-fold increase, respectively). In Experiment 2, an increase in the number of
hatchlings producing chimeric semen was detected after the homosexual transfer of
male-to-male compared with that after the heterosexual transfer of female-to-male
(68% to 88%). Significant increase was found in the frequency of chimeric semen production
(0.96 to 1.68 times); production of pheasant progenies by artificial insemination
using chimeric semen was also increased in the homosexual transfer (0 to 3 cases).
In conclusion, the homosexual PGC transfer of male-to-male yielded better rate of
generating pheasant progenies after test cross-reproduction than that of the heterosexual
transfer of female-to-male, which could improve the efficiency of interspecies germ
cell transfer system.