A list of bee species surveyed along an altitudinal gradient in Ubatuba, Brazil using Malaise and pan traps is presented. A total of 289 individuals representing 57 species, 33 genera, and 5 subfamilies were collected. Most individuals and species were collected at around 600 m altitude, and almost all species were collected with Malaise traps. Detrended Correspondence Analysis revealed a correlation between the occurrence of stingless bees and the altitude, confirming the usefulness of stingless-bee communities to reveal gradient patterns.