We have catalogued the protein kinase complement of the human genome (the "kinome")
using public and proprietary genomic, complementary DNA, and expressed sequence tag
(EST) sequences. This provides a starting point for comprehensive analysis of protein
phosphorylation in normal and disease states, as well as a detailed view of the current
state of human genome analysis through a focus on one large gene family. We identify
518 putative protein kinase genes, of which 71 have not previously been reported or
described as kinases, and we extend or correct the protein sequences of 56 more kinases.
New genes include members of well-studied families as well as previously unidentified
families, some of which are conserved in model organisms. Classification and comparison
with model organism kinomes identified orthologous groups and highlighted expansions
specific to human and other lineages. We also identified 106 protein kinase pseudogenes.
Chromosomal mapping revealed several small clusters of kinase genes and revealed that
244 kinases map to disease loci or cancer amplicons.