+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found

      Phosphofructokinase Activity as a Measure of Maturation of Rat Oocytes Developed in vivo and in vitro

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          We determined the activity of phosphofructokinase (PFK), a rate-limiting enzyme of glycolysis, in the rat oocyte during maturation either by luteinizing hormone stimulation in vivo or by cultivation under various conditions. In vivo, the activity of PFK in the maturing oocyte increased significantly, suggesting that the glycolytic pathway developed as the first meiotic division proceeded. A breakdown of the germinal vesicle and an increased activity of PFK were observed when denuded oocytes were cultured. Treatment of the oocytes with dibutyryl cAMP and 3-isobutyl-1-methyl xanthine almost completely inhibited germinal breakdown, whereas the activity of PFK increased significantly. The data suggest that regulation of PFK activity is not directly dependent on cAMP or cAMP-dependent protein kinase, and is not necessarily dependent on meiotic maturation. Our study also revealed that epidermal growth factor (EGF) significantly increased PFK activity, showing that EGF may be a potent inducer of oocyte maturation.

          Related collections

          Author and article information

          Hormone Research in Paediatrics
          S. Karger AG
          05 December 2008
          : 41
          : Suppl 1
          : 63-67
          Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Hongo, Tokyo, Japan
          183945 Horm Res 1994;41:63–67
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 5
          Local Regulators in the Ovary – Paracrine and Autocrine Control


          Comment on this article