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      Start Codon Targeted (SCoT) Polymorphism: A Simple, Novel DNA Marker Technique for Generating Gene-Targeted Markers in Plants

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      Plant Molecular Biology Reporter
      Springer Nature

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          TreeView: an application to display phylogenetic trees on personal computers.

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            Functional markers in plants.

            Different approaches (including association studies) have recently been adopted for the functional characterization of allelic variation in plants and to identify sequence motifs affecting phenotypic variation. We propose the term 'functional markers' for DNA markers derived from such functionally characterized sequence motifs. Functional markers are superior to random DNA markers such as RFLPs, SSRs and AFLPs owing to complete linkage with trait locus alleles. We outline the definition, development, application and prospects of functional markers.
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              AFLP genotyping and fingerprinting.

              Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP methods rapidly generate hundreds of highly replicable markers from DNA of any organism; thus, they allow high-resolution genotyping of fingerprinting quality. The time and cost efficiency, replicability and resolution of AFLPs are superior or equal to those of other markers [allozymes, random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), microsatellites], except that AFLP methods primarily generate dominant rather than co-dominant markers. Because of their high replicability and ease of use, AFLP markers have emerged as a major new type of genetic marker with broad application in systematics, pathotyping, population genetics, DNA fingerprinting and quantitative trait loci (QTL) mapping.
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                Author and article information

                Journal
                Plant Molecular Biology Reporter
                Plant Mol Biol Rep
                Springer Nature
                0735-9640
                1572-9818
                March 2009
                September 2008
                : 27
                : 1
                : 86-93
                Article
                10.1007/s11105-008-0060-5
                8b161764-ed5d-4db9-9968-4ba2889962c1
                © 2009
                Product
                Self URI (article page): http://link.springer.com/10.1007/s11105-008-0060-5

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