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      Development of a singleplex PCR assay for rapid identification and differentiation of Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, Cryptococcus gattii, and hybrids.

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          Abstract

          A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybrids were also characterized on the basis of distinct combined profiles of amplicons. This PCR assay is a rapid, simple, and reliable approach suitable for laboratory diagnoses and large-scale epidemiologic studies.

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          Author and article information

          Journal
          J. Clin. Microbiol.
          Journal of clinical microbiology
          American Society for Microbiology
          1098-660X
          0095-1137
          Jun 2013
          : 51
          : 6
          Affiliations
          [1 ] Medical Mycology Laboratory, Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
          Article
          JCM.00064-13
          10.1128/JCM.00064-13
          3716086
          23536400
          8b41eae9-d9bd-41c2-9450-8b4d47ee2bb2
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