Aquimarina longa sp. nov., isolated from seawater, and emended description of Aquimarina muelleri

Abstract

A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated SW024 ^T, was isolated from surface seawater of the South Pacific Gyre (26° 29′ S 137° 56′ W) during the Integrated Ocean Drilling Program Expedition 329. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina muelleri KMM 6020 ^T, Aquimarina macrocephali JAMB N27 ^T, Aquimarina addita JC2680 ^T, http://dx.doi.org/10.1601/nm.23278 Aquimarina mytili PSC33 ^T, Aquimarina intermedia KMM 6258 ^T, Aquimarina latercula ATCC 23177 ^T, Aquimarina spongiae A6 ^T, Aquimarina agarilytica ZC1 ^T and Aquimarina brevivitae SMK-19 ^T (96.1, 95.5, 95.0, 94.4, 94.3, 94.0, 93.4, 93.3 and 93.2 % similarities, respectively), demonstrating that the novel strain belonged to the genus Aquimarina . The DNA G+C content of strain SW024 ^T was 30.8 mol%. The major respiratory quinone of strain SW024 ^T was MK-6. The dominant fatty acids were iso-C _{15 : 0}, iso-C _{17 : 0} 3-OH, iso-C _{15 : 1} G, C _{16 : 0} 10-methyl and/or iso-C _{17 : 1}ω 9c, and iso-C _{15 : 0} 3-OH. The polar lipids of strain SW024 ^T were phosphatidylethanolamine, three unknown aminolipids and five unknown polar lipids. On the basis of combined phenotypic and phylogenetic analyses, strain SW024 ^T represents a novel species of the genus Aquimarina , for which the name Aquimarina longa sp. nov. is proposed. The type strain is SW024 ^T ( = CGMCC 1.11007 ^T = JCM 17859 ^T). An emended description of A. muelleri is also proposed.