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      A comparative analysis of attitudes toward stem cell research and regenerative medicine between six countries – A pilot study

      research-article
      a , ∗∗ , b , c , d ,
      Regenerative Therapy
      Japanese Society for Regenerative Medicine
      Regenerative medicine, Stem cell research, Science communication, International comparison, ELSI, RRI, AMED, Japan Agency for Medical Research and Development, ELSI, Ethical, Legal, and Social issues, iPSC, induced Pluripotent Stem Cell, ISSCR, International Society for Stem Cell Research, JST, Japan Science and Technology Agency, MEXT, The Japanese Ministry of Education, Sports, Culture, Science, and Technology, RiNCA, Responsible innovation with Conscience and Agility, RM, Regenerative Medicine, RRI, Responsible Research and Innovation, SCR, Stem Cell Research, UK, the United Kingdom, USA, the United States of America

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          Abstract

          Introduction

          Breakthroughs in stem cell research (SCR) and regenerative medicine (RM) have attracted significant public attention worldwide. Simultaneously, scientific communities and science policies have tried to establish appropriate governance of SCR and RM. In this context, effective communication between scientific communities and the public is regarded as a key factor. However, the diversity of public attitudes and interests has not been sufficiently examined, especially the differences across countries.

          Methods

          We conducted an international comparison of public attitudes toward SCR and RM. We circulated an internet questionnaire among people in six countries: Japan, South Korea, the United States, the UK, Germany, and France. We collected 100 valid responses from each country, and a total of 600 responses were obtained.

          Results

          Our key findings are the diversity of interests in RM, which can be expressed as user pragmatism, governance and handling of RM, risk, and benefit, and scientific interests. The priority of interests varied across the six countries, and the variations may be influenced by the political, social, cultural, and media contexts of SCR and RM in each country.

          Conclusion

          The implications can contribute to a deeper understanding of the diversity of public attitudes, and bring about an appropriate examination of a wide range of ethical and social concerns of SCR and RM in global contexts.

          Highlights

          • There are differences in public attitudes and expected RM across six countries.

          • There are types of interests in RM, such as user pragmatism, governance and handling of RM, etc.

          • Recognition of keywords of iPSCs is very high in Japan, comparing to other countries.

          • The trust of experts on RM are different across countries.

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          Most cited references23

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          Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.

          Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about factors that induce this reprogramming. Here, we demonstrate induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions. Unexpectedly, Nanog was dispensable. These cells, which we designated iPS (induced pluripotent stem) cells, exhibit the morphology and growth properties of ES cells and express ES cell marker genes. Subcutaneous transplantation of iPS cells into nude mice resulted in tumors containing a variety of tissues from all three germ layers. Following injection into blastocysts, iPS cells contributed to mouse embryonic development. These data demonstrate that pluripotent stem cells can be directly generated from fibroblast cultures by the addition of only a few defined factors.
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            Induction of pluripotent stem cells from adult human fibroblasts by defined factors.

            Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.
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              • Abstract: found
              • Article: not found

              Embryonic stem cell lines derived from human blastocysts.

              Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.
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                Author and article information

                Contributors
                Journal
                Regen Ther
                Regen Ther
                Regenerative Therapy
                Japanese Society for Regenerative Medicine
                2352-3204
                13 May 2022
                June 2022
                13 May 2022
                : 20
                : 187-193
                Affiliations
                [a ]Osaka University, Yamadaoka 2-8 Techni-Alliance Complex Build.C 611, Suita, Osaka 565-0871, Japan
                [b ]University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-0071, Japan
                [c ]Kanagawa University of Human Services, Japan, Research Gate Building TONOMACHI 2-A 2・3F, 3-25-10 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-0821, Japan
                [d ]Integrated Research Initiative for Living Well with Dementia, Tokyo Metropolitan Geriatric Medical Center, 35-2 Sakae-cho,Itabashi-ku, Tokyo, 173-0015, Japan
                Author notes
                []Corresponding author. Tokyo Metropolitan Geriatric Medical Center. yyashiro@ 123456keio.jp
                [∗∗ ]Corresponding author. Osaka University, Fax: +81-6-6105-6086. shineha@ 123456elsi.osaka-u.ac.jp
                Article
                S2352-3204(22)00036-0
                10.1016/j.reth.2022.04.007
                9114515
                35620641
                8b9eb194-ddf7-450c-ab3a-4d3e1f44f254
                © 2022 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                : 7 January 2022
                : 30 March 2022
                : 26 April 2022
                Categories
                Original Article

                regenerative medicine,stem cell research,science communication,international comparison,elsi,rri,amed, japan agency for medical research and development,elsi, ethical, legal, and social issues,ipsc, induced pluripotent stem cell,isscr, international society for stem cell research,jst, japan science and technology agency,mext, the japanese ministry of education, sports, culture, science, and technology,rinca, responsible innovation with conscience and agility,rm, regenerative medicine,rri, responsible research and innovation,scr, stem cell research,uk, the united kingdom,usa, the united states of america

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