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      Evidence that orexins A and B stimulate insulin secretion from rat pancreatic islets via both receptor subtypes.

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          Abstract

          Orexins are recently identified neuropeptides that appear to play a role in the regulation of energy homeostasis and arousal. They bind to and activate two closely related G protein-coupled receptors (OXR1 and OXR2), previously described as orphans. In this study we examined involvement of orexins in regulation of insulin secretion from rat pancreatic islets utilizing an in situ perfused pancreas and isolated pancreatic islet models. By means of RT-PCR we found that both OXR1 and OXR2 are expressed in rat pancreatic islets. Furthermore, the expression levels of OXR1 were higher than OXR2. In both experimental models applied, orexins A and B (1, 10 and 100 nmol/l) concentration dependently stimulated insulin secretion at two different glucose concentrations (6.66 or 26.4 mmol/l), with orexin A being more potent than orexin B. This study demonstrates that orexins A and B modulate insulin secretion in vitro.

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          Author and article information

          Journal
          Int. J. Mol. Med.
          International journal of molecular medicine
          1107-3756
          1107-3756
          Jun 2005
          : 15
          : 6
          Affiliations
          [1 ] Department of Animal Physiology and Biochemistry, August Cieszkowski University of Agriculture, 60-637 Poznan, Poland. kwnowak@owl.au.poznan.pl
          Article
          15870901

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