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      Unmanned Aerial Vehicles for High-Throughput Phenotyping and Agronomic Research

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          Abstract

          Advances in automation and data science have led agriculturists to seek real-time, high-quality, high-volume crop data to accelerate crop improvement through breeding and to optimize agronomic practices. Breeders have recently gained massive data-collection capability in genome sequencing of plants. Faster phenotypic trait data collection and analysis relative to genetic data leads to faster and better selections in crop improvement. Furthermore, faster and higher-resolution crop data collection leads to greater capability for scientists and growers to improve precision-agriculture practices on increasingly larger farms; e.g., site-specific application of water and nutrients. Unmanned aerial vehicles (UAVs) have recently gained traction as agricultural data collection systems. Using UAVs for agricultural remote sensing is an innovative technology that differs from traditional remote sensing in more ways than strictly higher-resolution images; it provides many new and unique possibilities, as well as new and unique challenges. Herein we report on processes and lessons learned from year 1—the summer 2015 and winter 2016 growing seasons–of a large multidisciplinary project evaluating UAV images across a range of breeding and agronomic research trials on a large research farm. Included are team and project planning, UAV and sensor selection and integration, and data collection and analysis workflow. The study involved many crops and both breeding plots and agronomic fields. The project’s goal was to develop methods for UAVs to collect high-quality, high-volume crop data with fast turnaround time to field scientists. The project included five teams: Administration, Flight Operations, Sensors, Data Management, and Field Research. Four case studies involving multiple crops in breeding and agronomic applications add practical descriptive detail. Lessons learned include critical information on sensors, air vehicles, and configuration parameters for both. As the first and most comprehensive project of its kind to date, these lessons are particularly salient to researchers embarking on agricultural research with UAVs.

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          Dissecting the phenotypic components of crop plant growth and drought responses based on high-throughput image analysis.

          Significantly improved crop varieties are urgently needed to feed the rapidly growing human population under changing climates. While genome sequence information and excellent genomic tools are in place for major crop species, the systematic quantification of phenotypic traits or components thereof in a high-throughput fashion remains an enormous challenge. In order to help bridge the genotype to phenotype gap, we developed a comprehensive framework for high-throughput phenotype data analysis in plants, which enables the extraction of an extensive list of phenotypic traits from nondestructive plant imaging over time. As a proof of concept, we investigated the phenotypic components of the drought responses of 18 different barley (Hordeum vulgare) cultivars during vegetative growth. We analyzed dynamic properties of trait expression over growth time based on 54 representative phenotypic features. The data are highly valuable to understand plant development and to further quantify growth and crop performance features. We tested various growth models to predict plant biomass accumulation and identified several relevant parameters that support biological interpretation of plant growth and stress tolerance. These image-based traits and model-derived parameters are promising for subsequent genetic mapping to uncover the genetic basis of complex agronomic traits. Taken together, we anticipate that the analytical framework and analysis results presented here will be useful to advance our views of phenotypic trait components underlying plant development and their responses to environmental cues. © 2014 American Society of Plant Biologists. All rights reserved.
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            Weed Mapping in Early-Season Maize Fields Using Object-Based Analysis of Unmanned Aerial Vehicle (UAV) Images

            The use of remote imagery captured by unmanned aerial vehicles (UAV) has tremendous potential for designing detailed site-specific weed control treatments in early post-emergence, which have not possible previously with conventional airborne or satellite images. A robust and entirely automatic object-based image analysis (OBIA) procedure was developed on a series of UAV images using a six-band multispectral camera (visible and near-infrared range) with the ultimate objective of generating a weed map in an experimental maize field in Spain. The OBIA procedure combines several contextual, hierarchical and object-based features and consists of three consecutive phases: 1) classification of crop rows by application of a dynamic and auto-adaptive classification approach, 2) discrimination of crops and weeds on the basis of their relative positions with reference to the crop rows, and 3) generation of a weed infestation map in a grid structure. The estimation of weed coverage from the image analysis yielded satisfactory results. The relationship of estimated versus observed weed densities had a coefficient of determination of r2=0.89 and a root mean square error of 0.02. A map of three categories of weed coverage was produced with 86% of overall accuracy. In the experimental field, the area free of weeds was 23%, and the area with low weed coverage (<5% weeds) was 47%, which indicated a high potential for reducing herbicide application or other weed operations. The OBIA procedure computes multiple data and statistics derived from the classification outputs, which permits calculation of herbicide requirements and estimation of the overall cost of weed management operations in advance.
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              Author and article information

              Contributors
              Role: Editor
              Journal
              PLoS One
              PLoS ONE
              plos
              plosone
              PLoS ONE
              Public Library of Science (San Francisco, CA USA )
              1932-6203
              29 July 2016
              2016
              : 11
              : 7
              : e0159781
              Affiliations
              [1 ]Department of Biological and Agricultural Engineering, Texas A&M University, College Station, Texas, 77843, United States of America
              [2 ]Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas, 77843, United States of America
              [3 ]Department of Aerospace Engineering, Texas A&M University, College Station, Texas, 77843, United States of America
              [4 ]Department of Geography, Texas A&M University, College Station, Texas, 77843, United States of America
              [5 ]LASERS Laboratory, Department of Ecosystem Science and Management, Texas A&M University, College Station, Texas, 77843, United States of America
              [6 ]Department of Mechanical Engineering, Texas A&M University, College Station, Texas, 77843, United States of America
              [7 ]Texas A&M AgriLife Research, Texas A&M University, College Station, Texas, 77843, United States of America
              [8 ]USDA-Agricultural Research Service, Aerial Application Technology Research Unit, 3103 F&B Road, College Station, Texas, 77845, United States of America
              New Mexico State University, UNITED STATES
              Author notes

              Competing Interests: The authors have declared that no competing interests exist.

              Conceived and designed the experiments: YS JAT SCM WLR NR CLM HLN MVB JV JO MPB SP DC BFM DDB. Performed the experiments: YS NAP SS GR JH EB RS EBP TB CY. Analyzed the data: YS SCM NAP SS NR GR HLN AR MVB JO MPB RS EBP. Contributed reagents/materials/analysis tools: AI CY. Wrote the paper: YS JAT SCM NAP NR HLN MVB JV RS EBP. Administrative management: RVA MV.

              Article
              PONE-D-16-11279
              10.1371/journal.pone.0159781
              4966954
              27472222
              8be06823-8706-47a1-bc5f-166d4d251791

              This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.

              History
              : 17 March 2016
              : 6 July 2016
              Page count
              Figures: 15, Tables: 4, Pages: 26
              Funding
              This project was supported by Texas A&M AgriLife Research, the Texas Engineering Experiment Station, Texas A&M Center for Geospatial Sciences, Applications and Technology (GEOSAT), and Texas A&M Center for Autonomous Vehicles and Sensor Systems (CANVASS). Field research projects were supported by USDA Hatch funds and other funding to individual investigators.
              Categories
              Research Article
              Biology and Life Sciences
              Organisms
              Plants
              Weeds
              Biology and Life Sciences
              Agriculture
              Agricultural Soil Science
              Ecology and Environmental Sciences
              Soil Science
              Agricultural Soil Science
              Biology and Life Sciences
              Agriculture
              Agronomy
              Engineering and Technology
              Remote Sensing
              Biology and Life Sciences
              Agriculture
              Farms
              Biology and Life Sciences
              Agriculture
              Agronomy
              Plant Breeding
              Engineering and Technology
              Aerospace Engineering
              Flight Testing
              Engineering and Technology
              Equipment
              Optical Equipment
              Cameras
              Custom metadata
              All relevant data are within the paper and its Supporting Information files deposited in Dryad digital repository with the URL/DOI of ( http://datadryad.org/review?doi=doi:10.5061/dryad.65m87).

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              Uncategorized

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