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      Correction: Genetic Interactions between the Members of the SMN-Gemins Complex in Drosophila

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          Abstract

          There are multiple errors in Table 1. There is no colour separation between the 'Gemin3 x Gemin5' and 'Gemin3 x SMN' sections. The delta symbol is missing in the Gem3BART + Gem5ΔN cell of column 2. The publisher apologizes for the error. Please see the corrected Table 1 here: 10.1371/journal.pone.0139649.t001 Table 1 Summary of the phenotypic effects resulting from all genetic manipulations. Genetic Interaction Genotype: Mef2-GAL4> Adult Viable Motor Defects Gemin3 x Gemin5 Gem3 BART Yes No Gem3 BART X2 Yes Yes Gem5 FL Yes No Gem3 BART + Gem5 FL Yes Yes Gem5 ∆N Yes No Gem3 BART + Gem5 ∆N Yes Yes Gem5-IR nan+sac Yes No Gem3 BART + Gem5-IR nan+sac No N/A Df(2R)exu1 Yes No Gem3 BART + Df(2R)exu1 Yes Yes Gemin3 x SMN Gem3 BART + Smn X7 Yes No GFP-Smn FL Yes No Gem3 BART + GFP-Smn FL No N/A GFP-Smn ∆6 Yes No Gem3 BART + GFP-Smn ∆6 Yes Yes Flag-Smn FL Yes No Gem3 BART + Flag-Smn FL Semi Yes Smn-IR FL26B Yes No Gem3 BART + Smn-IR FL26B No N/A Smn-IR N4 Yes No Gem3 BART + Smn-IR N4 No N/A Smn-IR C24 Yes No Gem3 BART + Smn-IR C24 Yes Yes Gemin3 x Gemin2 Gem3 BART + Df(3L)ED4782 Yes No Gem2 FL Yes Yes Gem3 BART + Gem2 FL No N/A Gem2 ∆C Yes No Gem3 BART + Gem2 ∆C Yes Yes Gem2 ∆N Yes No Gem3 BART + Gem2 ∆N No N/A Gem2-IR gau Yes No Gem3 BART + Gem2-IR gau Semi Yes Gem2-IR gau X2 Yes No Gem3 BART + Gem2-IR gau X2 No N/A

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          Genetic Interactions between the Members of the SMN-Gemins Complex in Drosophila

          The SMN-Gemins complex is composed of Gemins 2–8, Unrip and the survival motor neuron (SMN) protein. Limiting levels of SMN result in the neuromuscular disorder, spinal muscular atrophy (SMA), which is presently untreatable. The most-documented function of the SMN-Gemins complex concerns the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs). Despite multiple genetic studies, the Gemin proteins have not been identified as prominent modifiers of SMN-associated mutant phenotypes. In the present report, we make use of the Drosophila model organism to investigate whether viability and motor phenotypes associated with a hypomorphic Gemin3 mutant are enhanced by changes in the levels of SMN, Gemin2 and Gemin5 brought about by various genetic manipulations. We show a modifier effect by all three members of the minimalistic fly SMN-Gemins complex within the muscle compartment of the motor unit. Interestingly, muscle-specific overexpression of Gemin2 was by itself sufficient to depress normal motor function and its enhanced upregulation in all tissues leads to a decline in fly viability. The toxicity associated with increased Gemin2 levels is conserved in the yeast S. pombe in which we find that the cytoplasmic retention of Sm proteins, likely reflecting a block in the snRNP assembly pathway, is a contributing factor. We propose that a disruption in the normal stoichiometry of the SMN-Gemins complex depresses its function with consequences that are detrimental to the motor system.
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            Journal
            PLoS One
            PLoS ONE
            plos
            plosone
            PLoS ONE
            Public Library of Science (San Francisco, CA USA )
            1932-6203
            25 September 2015
            2015
            : 10
            : 9
            : e0139649
            Article
            PONE-D-15-41061
            10.1371/journal.pone.0139649
            4583472
            26406611
            8c16a1a5-5df5-48b0-a5bf-9d727d5a7fcf
            Copyright @ 2015

            This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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