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      Survey of chicken abattoir for the presence of Campylobacter jejuni and Campylobacter coli Translated title: Pesquisa de Campylobacter jejuni e Campylobacter coli em abatedouros de aves

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          Abstract

          The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhea. These species may be found in water, food and in the intestinal tract of chickens. This study investigated the presence of Campylobacter jejuni and Campylobacter coli in chicken abattoirs in São Paulo State, Brazil. A total of 288 samples of feces, feathers, scald water, evisceration water, chiller water, and the rinse water of eviscerated, not eviscerated and chilled carcasses were collected in six chicken abattoirs. Polymerase Chain Reaction (PCR) was performed in Campylobacter spp.-positive isolates using the gene HIP, specific for hippuricase enzyme from Campylobacter jejuni and aspartokinase gene, specific to detect Campylobacter coli. The percentage of positive isolates of Campylobacter jejuni was 4.9% (14/288). Isolation was greater in feces samples (22%, 8/36). One sample was positive for the species C. coli. In conclusion, the results indicate that it is necessary to improve quality control for Campylobacter spp. in chicken abattoirs.

          Translated abstract

          O gênero Campylobacter tem grande destaque em saúde pública, principalmente por pertencerem a este gênero várias espécies que podem causar diarréia. Estas espécies podem ser encontradas em amostras de água, alimentos e no trato intestinal das aves. Este estudo investigou a presença de Campylobacter jejuni e Campylobacter coli em abatedouros de aves no Estado de São Paulo. As 288 amostras foram coletadas em seis estabelecimentos e incluíram: fezes; penas; água de escaldamento, de evisceração e de resfriamento; e água de enxaguadura de carcaça não eviscerada, eviscerada e resfriada. Após o isolamento microbiológico das amostras positivas de Campylobacter spp. foi realizada uma Reação em Cadeia da Polimerase (PCR) utilizando o gene HIP, da hipuricase, específico para Campylobacter jejuni e o gene da enzima aspartoquinase, específico para Campylobacter coli. A porcentagem de amostras positivas para Campylobacter spp. foi de 4,9% (14/288), sendo que o isolamento foi maior em amostras de fezes (22%, 8/36). Foi isolada uma amostra positiva para C. coli. Em conclusão, os resultados indicam que há uma necessidade de melhorar a qualidade higiênico-sanitária do controle de Campylobacter em abatedouros de aves.

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          Most cited references28

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          Relationship between aerobic bacteria, salmonellae and Campylobacter on broiler carcasses

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            Introduction and spread of thermophilic campylobacters in broiler flocks.

            S J Evans (2016)
            Campylobacteriosis is the most commonly reported infectious cause of human gastroenteritis in developed countries and broiler chickens are considered to be the major food-borne source of the infection. The control of the infection in man depends upon its control in broiler flocks but the epidemiology in poultry is poorly understood. Up to 50 per cent of broiler flocks may be infected and most of the birds in an infected flock carry the organisms until slaughter. Vertical transmission through the egg appears unlikely but there are many other potential sources of the infection for the chicks; direct contact with infected animals or birds has been proposed and there is also evidence for indirect transmission through drinking water or insect vectors. It is suggested that the cross-sectional studies discussed in this review should be followed by well designed case-control studies to test the aetiological hypotheses put forward.
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              Genome sequence-based fluorescent amplified fragment length polymorphism of Campylobacter jejuni, its relationship to serotyping, and its implications for epidemiological analysis.

              The published genome sequence of Campylobacter jejuni strain NCTC 11168 was used to model an accurate and highly reproducible fluorescent amplified fragment length polymorphism (FAFLP) analysis. Predicted and experimentally observed amplified fragments (AFs) generated with the primer pair HindIII+A and HhaI+A were compared. All but one of the 61 predicted AFs were reproducibly detected, and no unpredicted fragments were amplified. This FAFLP analysis was used to genotype 74 C. jejuni strains belonging to the nine heat-stable (HS) serotypes most prevalent in human disease in England and Wales. The 74 C. jejuni strains exhibited 60 FAFLP profiles, and cluster analysis of them yielded a radial tree showing genetic relationships between and within 13 major clusters. Some clusters were related, and others were unrelated, to a single HS serotype. For example, all strains belonging to serotypes HS6 and HS19 grouped into corresponding single genotypic clusters, while strains of serotypes HS11 and HS18 each grouped into two genotypic clusters. Strains of HS50, the most prevalent serotype infecting humans, were found both in one large (multiserotype) cluster complex and dispersed throughout the tree. The strain genotypes within each FAFLP cluster were characterized by a particular combination of AFs, and among the cluster there were additional differential AFs. Identification of such AFs could act as a search tool to look for potential associations with disease or animal hosts, when applied to large number of human isolates. Genome-sequence based FAFLP, thus, has the potential to establish a genetic database for epidemiological investigations of Campylobacter.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rimtsp
                Revista do Instituto de Medicina Tropical de São Paulo
                Rev. Inst. Med. trop. S. Paulo
                Instituto de Medicina Tropical (São Paulo )
                1678-9946
                December 2006
                : 48
                : 6
                : 307-310
                Affiliations
                [1 ] Universidade Estadual Paulista Brazil
                [2 ] Instituto Biológico de São Paulo Brazil
                Article
                S0036-46652006000600001
                10.1590/S0036-46652006000600001
                8c213bf7-9b61-4519-95f3-6725bd5a8fe3

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0036-4665&lng=en
                Categories
                TROPICAL MEDICINE

                Infectious disease & Microbiology
                Abattoir,Chicken,PCR,Campylobacter coli,Campylobacter jejuni
                Infectious disease & Microbiology
                Abattoir, Chicken, PCR, Campylobacter coli, Campylobacter jejuni

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