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Expression and assay of HECT domain ligases.

Author(s): J M Huibregtse, Anahita Dastur, Sylvie Beaudenon

Publication date: 2004-12-31

Keywords: Endosomal Sorting Complexes Required for Transport, Humans, Protein Structure, Tertiary, Saccharomyces cerevisiae, enzymology, genetics, Saccharomyces cerevisiae Proteins, analysis, biosynthesis, Ubiquitin-Conjugating Enzymes, metabolism, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases

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Abstract

HECT domain ubiquitin ligases (HECT E3s), typified by human E6AP and yeast Rsp5p, are unique among the several classes of known ubiquitin ligases in that they participate directly in the chemistry of substrate ubiquitination reactions. This chapter discusses strategies for the expression of active HECT E3s and the assays that are available for analyzing E2 interaction, ubiquitin-thioester formation, and substrate ubiquitination.

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PubMed ID:: 16275324

DOI:: 10.1016/S0076-6879(05)98011-7

ScienceOpen disciplines: Chemistry

Keywords: Endosomal Sorting Complexes Required for Transport,Humans,Protein Structure, Tertiary,Saccharomyces cerevisiae,enzymology,genetics,Saccharomyces cerevisiae Proteins,analysis,biosynthesis,Ubiquitin-Conjugating Enzymes,metabolism,Ubiquitin-Protein Ligase Complexes,Ubiquitin-Protein Ligases

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ScienceOpen disciplines: Chemistry

Expression and assay of HECT domain ligases.

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