16
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Método analítico para a determinação de meloxicam em plasma humano por cromatografia líquida de alta eficiência (CLAE) Translated title: Determination of meloxicam in human plasma by HPLC

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Desenvolveu-se e validou-se método analítico simples, rápido e específico para quantificação de meloxicam (inibidor da COX-2) em plasma humano através da cromatografia líquida de alta eficiência, para aplicação em estudos de bioequivalência. Piroxicam foi utilizado como padrão interno. Empregou-se cromatografia em fase reversa com coluna modelo Synergi RP-MAX (150 x 4,6 mm), à temperatura de 30 ºC e fase móvel constituída por mistura de acetonitrila e tampão fosfato 0,025 mol/L pH 4,5 (40:60, v/v), a um fluxo de 1,0 mL/min. Os analitos foram detectados por UV a 364 nm. As amostras de plasma foram acidificadas com ácido clorídrico 1 mol/L, extraídas utilizando-se éter terc-butil metílico e, após filtração e secagem, o resíduo foi reconstituído em 250 mL de fase móvel para injeção em CLAE. Os tempos de retenção para meloxicam (padrão) e piroxicam (padrão interno) foram 3,35 e 4,19 minutos, respectivamente. Este método apresentou linearidade na faixa de concentração entre 50-4000 ng/mL (R² = 0,9995), com limite de quantificação inferior de 50 ng/mL e exatidão de 114%. O método analítico desenvolvido neste trabalho demonstrou especificidade, linearidade, precisão e exatidão adequadas, permitindo sua aplicação em ensaios de bioequivalência.

          Translated abstract

          A simple, rapid and specific high-performance liquid cromatographic (HPLC) method was developed and validated to estimate meloxicam (COX-2 inhibitor) levels in human plasma. Piroxicam was used as internal standard. Reversed phase chromatography was conducted using a Synergi RP-MAX (150 x 4.6 mm) column at 30 ºC and a mobile phase of acetonitrile and 0.025 mol/L pH 4,5 phosphate buffer (40:60, v/v), at a flow rate of 1mL/min. Analytes were detected at 364 nm. Plasma samples were acidified with 1 mol/L hydrochloric acid and extracted with tert-butyl methyl ether, evaporated to dryness, reconstituted in 250 mL of mobile phase and injected in the column. The retention time of meloxicam and piroxicam were 3.35 and 4.19 minutes, respectively. This method showed to be linear in the range of 50 - 4500 ng/mL (R² = 0.9995), a LOQ of 50 ng/mL and accuracy of 114%. The analytical method showed suitable specificity, sensitivity, linearity, precision and accuracy, and can be used in bioequivalence or pharmacokinetics studies involving meloxicam.

          Related collections

          Most cited references11

          • Record: found
          • Abstract: found
          • Article: not found

          Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion.

          R Causon (1997)
          Bioanalytical method validation is the process used to establish that a quantitative analytical method is suitable for biomedical applications. Reassurances as to the quality of the method and its reliability come from adopting a minimum series of validation experiments and obtaining satisfactory results. Consistent evaluation of the key analytical parameters: recovery, response function, sensitivity, precision, accuracy, selectivity and stability, is discussed with a view to improving scientific standards in manuscripts submitted for publication.
            Bookmark
            • Record: found
            • Abstract: not found
            • Book: not found

            Dicionário Terapêutico Guanabara

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Resolução nº 899, de 29 de maio de 2003

              (2003)
                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rbcf
                Revista Brasileira de Ciências Farmacêuticas
                Rev. Bras. Cienc. Farm.
                Divisão de Biblioteca e Documentação do Conjunto das Químicas da Universidade de São Paulo (São Paulo )
                1516-9332
                June 2005
                : 41
                : 2
                : 215-222
                Affiliations
                [1 ] Universidade de São Paulo Brazil
                Article
                S1516-93322005000200009
                10.1590/S1516-93322005000200009
                8c7a6d02-0a17-421a-8497-95818519286e

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1516-9332&lng=en
                Categories
                PHARMACOLOGY & PHARMACY

                Pharmacology & Pharmaceutical medicine
                Meloxicam,Piroxicam,Plasma,HPLC,Plasma humano,Cromatografia líquida de alta eficiência (CLAE)

                Comments

                Comment on this article