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      Effects of aeration, agitation and pH on the production of mycelial biomass and exopolysaccharide from the filamentous fungus Ganoderma lucidum Translated title: Efecto de la aireación, la agitación y el pH sobre la producción de biomasa micelial y exopolisacáridos del hongo filamentoso Ganoderma lucidum

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          Abstract

          Abstract Due to the increasing demand for functional foods and dietary supplements, mushroom production has increased significantly in recent years, which is why the study of operational conditions affecting the biotechnology cultivation of these mushrooms has become the aim of numerous studies. This study attempted to know the effect of pH, agitation and aeration on the biomass and polysaccharides production of filamentous fungus Ganoderma lucidum in fermentations made in a 5L bioreactor. The results allowed to establish that a bistage pH strategy (one stage with a fixed pH control, and a second stage without control), combined with hydrodynamic stress, which involves high agitation and high aeration, favors biomass and polysaccharides production, achieving increases of up to 100% compared to that obtained with the reference conditions.

          Translated abstract

          Resumen Debido a la creciente demanda de alimentos funcionales y suplementos dietarios, la producción de hongos ha aumentado significativamente en los últimos años, razón por la cual el estudio de las condiciones operacionales que afectan el cultivo biotecnológico de estos hongos se ha convertido en el objeto de estudio de muchos investigadores. Con esta investigación se busca evaluar el efecto que tiene el pH, la agitación y la aireación sobre la producción de biomasa y polisacáridos de un hongo filamentoso en fermentaciones realizadas en biorreactor de 5L. Los resultados permitieron establecer que una estrategia de pH escalonado que incluye dos etapas una con control a un pH fijo y una segunda etapa sin control, combinada con estrés hidrodinámico, que implica alta agitación y alta aireación, favorece la producción de biomasa y polisacáridos, alcanzando incrementos de hasta 100% comparados con los obtenido con las condiciones de referencia.

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          Effect of fatty acids on the mycelial growth and polysaccharide formation by Ganoderma lucidum in shake flask cultures

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            Colorimetric method for determination of sugars and related substances

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              Use of dinitosalicylic acid form determination of reducing sugars

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                dyna
                DYNA
                Dyna rev.fac.nac.minas
                Universidad Nacional de Colombia (Medellín, Antioquia, Colombia )
                0012-7353
                March 2017
                : 84
                : 200
                : 72-79
                Affiliations
                [2] Medellín Antioquía orgnameUniversidad de Antioquia Colombia ygl0317@ 123456gmail.edu.co
                [3] Medellín Antioquía orgnameUniversidad de Antioquia Colombia urrego.sergio@ 123456gmail.com
                [1] Medellín Antioquía orgnameUniversidad de Antioquia Colombia
                [4] Medellín orgnameCEO, Progal BT Colombia direccionejecutiva@ 123456progal-bt.com
                Article
                S0012-73532017000100072
                10.15446/dyna.v84n200.57126
                8c86d4ce-6751-4a23-9794-9c8ee09765c2

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 23 April 2016
                : 09 November 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 11, Pages: 8
                Product

                SciELO Colombia


                fungi submerged culture,Exopolysaccharides,Mycelial fungi,bioreactor operation,Ganoderma lucidum,Cultivo biotecnológico,cultivo sumergido de hongos,exopolisacáridos,Hongo miceliar,operación de biorreactores,Biotechnological culture

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