Colonic Expression of Genes Encoding Inflammatory Mediators and Gelatinases During Campylobacter Jejuni Infection of Conventional Infant Mice

Abstract

Within 1 week following peroral Campylobacter jejuni infection, infant mice develop acute enteritis resolving thereafter. We here assessed colonic expression profiles of mediators belonging to the IL-23/IL-22/IL-18 axis and of matrix-degrading gelatinases MMP-2 and MMP-9 at day 6 post C. jejuni strain 81-176 infection. Whereas the pathogen readily colonized the intestines of infant IL-18 ^–/– mice only, colonic mucin-2 mRNA, a pivotal mucus constituent, was downregulated in IL-22 ^–/– mice and accompanied by increased expression of pro-inflammatory cytokines including IFN-γ, TNF, IL-17A, and IL-1β. Furthermore, in both naive and infected IL-22 ^–/– mice, colonic expression of IL-23p19 and IL-18 was lower as compared to wildtype mice, whereas, conversely, colonic IL-22 mRNA levels were lower in IL-18 ^–/– and colonic IL-18 expression lower in IL-23p19 ^–/– as compared to wildtype mice. Moreover, colonic expression of MMP-2 and MMP-9 and their endogenous inhibitor TIMP-1 were lower in IL-22 ^–/– as compared to wildtype mice at day 6 postinfection. In conclusion, mediators belonging of the IL-23/IL-22/IL-18 axis as well as the gelatinases MMP-2 and MMP-9 are involved in mediating campylobacteriosis of infant mice in a differentially regulated fashion.

Journal

Journal ID (nlm-ta): Eur J Microbiol Immunol (Bp)

Journal ID (iso-abbrev): Eur J Microbiol Immunol (Bp)

Journal ID (publisher-id): EUJMI

Title: European Journal of Microbiology & Immunology

Publisher: Akadémiai Kiadó (Budapest )

ISSN (Print): 2062-509X

ISSN (Electronic): 2062-8633

Publication date (Electronic): 25 April 2016

Publication date Collection: 24 June 2016

Volume: 6

Issue: 2

Pages: 137-146

Affiliations

Department of Microbiology and Hygiene, Charité – University Medicine Berlin , Berlin, Germany

Author notes

* Charité – University Medicine Berlin, CC5, Department of Microbiology and Hygiene, Campus Benjamin Franklin, FEM, Garystr. 5, D-14195 Berlin, Germany; [phone]; markus.heimesaat@ 123456charite.de

Financial disclosure and grant support

This work was supported by grants from the German Research Foundation (DFG) to A.F., S.B., and U.B.G. (SFB633, TP A7); M.M.H. (SFB633, TP B6); and A.E.K. and U.G. (SFB633, Immuco), and from the German Federal Ministry of Education and Research (BMBF) to S.B. (TP1.1).

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the article.

Competing interests

The authors declare that no competing interests exist.

Article

DOI: 10.1556/1886.2016.00009

PMC ID: 4936336

PubMed ID: 27429796

Copyright statement: © The Author(s)

License:

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Counts

Figures: 10, Tables: 0, Equations: 0, References: 32, Pages: 10

Categories

Subject: Original Article