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      Seroepidemiological survey of Rickettsia spp. in dogs from the endemic area of Rickettsia parkeri rickettsiosis in Uruguay.

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          Abstract

          Rickettsia parkeri rickettsiosis is a vector-borne zoonosis that occurs in some countries of the American continent. Following the first description and determination of the pathogenicity to humans in 2004 in USA, this bacterium has been reported in several South American countries. Human cases have been diagnosed in both Uruguay and Argentina in the past years. This study consisted in a serosurvey of 1000 domestic dogs living in the endemic area of rickettsiosis in Uruguay, where Amblyomma triste is the tick vector. Sera were analyzed by Indirect Immunofluorescence Assay (IFA), against antigens of three different rickettsial species: R. rhipicephali, R. felis and R. parkeri. It was determined that 20.3% of the dogs had antibodies that reacted to at least one of the three species tested, taking as cut off ≥64 titers. Furthermore, 140 of the seropositive dogs (14%) had a titer at least 4 times higher to R. parkeri than those of any of the other species, thus, it was considered that the immune response was stimulated by that species in particular. This is the first serological survey in primary hosts for adults of A. triste in Uruguay, and therefore the first prevalence values are reported. Adult A. triste ticks collected from the environment as well as from dogs were analyzed by PCR in order to confirm the current circulation of the agent in the area. In this matter, two out of 28 ticks from dogs, and 3 out of 53 ticks from the environment were positive, and the corresponding sequence analysis revealed 100% similarity with R. parkeri strain maculatum.

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          Author and article information

          Journal
          Acta Trop.
          Acta tropica
          1873-6254
          0001-706X
          Jun 2015
          : 146
          Affiliations
          [1 ] Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, Av. Alberto Lasplaces 1620, Montevideo CP 11600, Uruguay. Electronic address: pau.parasito@gmail.com.
          [2 ] Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Av. Prof. Orlando M. de Paiva 87, São Paulo 05508-900, Brazil.
          [3 ] Departamento de Biología Celular y Molecular, Facultad de Veterinaria, Universidad de la República, Av. Alberto Lasplaces 1620, Montevideo CP 11600, Uruguay. Electronic address: jmverdes@fvet.edu.uy.
          [4 ] Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Av. Prof. Orlando M. de Paiva 87, São Paulo 05508-900, Brazil. Electronic address: labruna@usp.br.
          [5 ] Laboratorio de Vectores y enfermedades transmitidas and Departamento de Parasitología Veterinaria, Facultad de Veterinaria, Universidad de la República, CENUR Noroeste - Salto, Rivera 1350, Salto CP 50000, Uruguay. Electronic address: dpvuru@hotmail.com.
          Article
          S0001-706X(15)00042-X
          10.1016/j.actatropica.2015.02.014
          25735816
          Copyright © 2015 Elsevier B.V. All rights reserved.

          Uruguay, Amblyomma triste, Dogs, Rickettsia parkeri, Serosurvey

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