Studies of infectious diseases have been limited by the lack of models that recapitulate
normal cellular physiology and pathology. Developments in organotypic models have
paved the road towards further studies of viral infections and host–virus interactions.
For example, human intestinal organoids were efficiently used to study many viruses,
such as rotavirus, norovirus, enterovirus 71, and human adenovirus.
1
Mammalian airway organoids are complex three-dimensional structures characterised
by different cellular composition and designed to mimic lung structures. Early research
attempted to develop these organoids from different progenitor cells, including basal
cells, secretory cells, and alveolar epithelial cells.
2
In the past 5 years, scientists were able to generate mature lung organoids that contain
basal, ciliated, and club cells. These organoids were used to study diseases such
as cystic fibrosis and lung tumours, and infections.
3
One study
4
used airway organoids to look at viral replication, tissue tropism, and immune response
to many human influenza A and avian viruses.
Fortunately, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus
responsible for COVID-19, was isolated and propagated early on in the pandemic using
numerous in-vitro models, such as Vero cells, Huh7 cells, and human airway epithelial
cells. This isolation was enhanced after SARS-CoV-2 was isolated and propagated in
TMPRSS2-expressing VeroE6 cells, indicating the vital role of TMPRSS2 serine protease
in virus infectivity.
5
Thus, in-vitro models are effective in the study of virus propagation, but they poorly
recapitulate respiratory tract histology and function.
We recommend the use of human airway organoids as a model to study SARS-CoV-2 replication
kinetics, tropism, and host response (figure
). Airway organoids can be generated using healthy lung tissue derived from patients
undergoing surgical resection, and SARS-CoV-2 can be obtained from clinical specimens
from patients who have tested positive. After the airway organoid is infected with
SARS-CoV-2, immunofluorescence and electron scanning microscopes could be used to
study the cytopathic effects of viral particles on different cell types. Furthermore,
whole-genome sequencing and real-time quantitative PCR could determine viral replication
kinetics and genetic alterations, and transcriptomic profiling could reveal the differential
expression of genes related to viral infection. Additionally, flow cytometry enables
the detection and quantification of different cell types before and after SARS-CoV-2
infection.
Figure
Co-culture of airways organoids
Co-culture of airways organoids with SARS-CoV-2 could be used to study viral replication,
tropism, and pathogenicity in addition to structural changes (A); study immune responses
and cytokine release, recapitulate some pathological conditions such as cytokine release
syndrome, and develop immunomodulatory drugs (B); and as a tool for antiviral drug
discovery and development (C). Created using BioRender.com.
Angiotensin converting enzyme 2 and TMPRSS2 serine protease are highly expressed in
human airway epithelia and airway organoids,6, 7 making models that use airway organoids
suitable for the study of viral infectivity, since these proteins are thought to facilitate
infection of cells.
Airway organoids could also be used in a co-culture model and be cultured with different
immune cells. This co-culture model would enable the study of immunological responses
to SARS-CoV-2. Moreover, genomic and transcriptomic profiling could reveal further
signalling pathways involved in such immune responses. It is also possible to detect
the secreted cytokines in response to SARS-CoV-2 infection and hence provide a model
to recapitulate cytokine release syndrome seen in some patients with COVID-19.
8
Moreover, a co-culture model could be used to explore the activity of immunomodulatory
drugs.
Airway organoids could also be used to discover effective antiviral drugs to treat
COVID-19. The potential activity of drug candidates could be predicted by several
laboratory methods: real-time quantitative PCR can assess viral load, while immunofluorescence
and electron microscopy can identify the number of cells that have been infected.
Additionally, microarray analyses can identify the molecular mechanisms of investigational
drugs and their possible cellular targets. Models using airway organoids could be
invaluable to learn more about SARS-CoV-2 infectivity, replication kinetics, and host–virus
interactions, an understanding of which will be key to help fight the current pandemic.