Blog
About

9
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Antcin K, an active triterpenoid from the fruiting bodies of basswood cultivated Antrodia cinnamomea, induces mitochondria and endoplasmic reticulum stress-mediated apoptosis in human hepatoma cells

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Liver cancer is the second leading cause of cancer deaths in Taiwan as per the 2011 statistics and ranks fourth in cancer-related mortality in the world. Recent researches have shown that Antrodia cinnamomea, a Taiwan-specific medicinal mushroom, has biological activities, including hepatoprotection, anti-inflammation, antihepatitis B virus activity, and anticancer activity. In the present study, the antiproliferative activity and molecular mechanisms of antcin K, the most abundant ergostane triterpenoid from the fruiting bodies of basswood cultivated A. cinnamomea, were investigated using human hepatoma Hep 3B cells. The results showed that antcin K effectively reduced Hep 3B cells viability within 48 hours. Antcin K induced phosphatidylserine exposure, chromatin condensation, and DNA damage, but did not significantly increase autophagosome content or cause cell expansion and cell lysis. Thus, the principal mode of Hep 3B cells death induced by antcin K was apoptosis, rather than autophagy or necrosis. In-depth investigation of the molecular mechanisms revealed that antcin K first promoted reactive oxygen species generation and adenosine triphosphate depletion, leading to endoplasmic reticulum stress and resulting in mitochondrial membrane permeability changes. After losing the mitochondrial membrane potential, caspase-independent and caspase-dependent apoptosis-related proteins were released, including HtrA 2, apoptotic-induced factor, endonuclease G, and cytochrome c. Cytochrome c activated caspase-9 and caspase-3, and cut downstream protein PARP, ultimately leading to cell apoptosis. These results suggested that antcin K induced mitochondrial and endoplasmic reticulum stress-mediated apoptosis in human hepatoma cells. Coupled with these findings, antcin K has a potential to be a complementary agent in liver cancer therapy.

          Related collections

          Most cited references 41

          • Record: found
          • Abstract: not found
          • Article: not found

          A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays

            A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation. The assay detects living, but not dead cells and the signal generated is dependent on the degree of activation of the cells. This method can therefore be used to measure cytotoxicity, proliferation or activation. The results can be read on a multiwell scanning spectrophotometer (ELISA reader) and show a high degree of precision. No washing steps are used in the assay. The main advantages of the colorimetric assay are its rapidity and precision, and the lack of any radioisotope. We have used the assay to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Self-eating and self-killing: crosstalk between autophagy and apoptosis.

              The functional relationship between apoptosis ('self-killing') and autophagy ('self-eating') is complex in the sense that, under certain circumstances, autophagy constitutes a stress adaptation that avoids cell death (and suppresses apoptosis), whereas in other cellular settings, it constitutes an alternative cell-death pathway. Autophagy and apoptosis may be triggered by common upstream signals, and sometimes this results in combined autophagy and apoptosis; in other instances, the cell switches between the two responses in a mutually exclusive manner. On a molecular level, this means that the apoptotic and autophagic response machineries share common pathways that either link or polarize the cellular responses.
                Bookmark

                Author and article information

                Affiliations
                [a ]Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan
                [b ]Department of Food Science, Rutgers University, New Brunswick, NJ, USA
                [c ]Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan
                [d ]Department of Biotechnology, Asia University, Taichung, Taiwan
                [e ]National Center for Food Safety Education and Research, National Taiwan University, Taipei, Taiwan
                [f ]Center for Food and Biomolecules, National Taiwan University, Taipei, Taiwan
                [g ]International Mater's Degree Program in Food Science, International College, National Pingtung University of Science and Technology, 1 Shuefu Road, Neipu, Pingtung 91201, Taiwan
                Author notes
                []Corresponding author. National Taiwan University, Number 1, Section 4, Roosevelt Road, Taipei 106, Taiwan. lysheen@ 123456ntu.edu.tw
                [∗∗ ]Corresponding author. China Medical University, Hsueh-Shih Road, Taichung, 404, Taiwan, Republic of China. kuoyh@ 123456mail.cmu.edu.tw
                Contributors
                Journal
                J Tradit Complement Med
                J Tradit Complement Med
                Journal of Traditional and Complementary Medicine
                Elsevier
                2225-4110
                04 February 2015
                January 2016
                04 February 2015
                : 6
                : 1
                : 48-56
                26870680 4737972 S2225-4110(14)00040-6 10.1016/j.jtcme.2014.11.026
                Copyright © 2016, Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC. All rights reserved.

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

                Categories
                Original Article

                Comments

                Comment on this article