Understanding the patterns and drivers of carbon isotope discrimination in C 4 species is critical for predicting the effects of global change on C 3/C 4 ratio of plant community and consequently on ecosystem functioning and services. Cleistogenes squarrosa (Trin.) Keng is a dominant C 4 perennial bunchgrass of arid and semi-arid ecosystems across the Mongolian plateau of the Eurasian steppe. Its carbon isotope discrimination ( 13 Δ) during photosynthesis is relatively large among C 4 species and it is variable. Here the 13 Δ of C. squarrosa and its potential drivers at a nested set of scales were examined. Within cohorts of tillers, 13 Δ of leaves increased from 5.1‰ to 8.1‰ from old to young leaves. At the local scale, 13 Δ of mature leaves varied from 5.8‰ to 8.4‰, increasing with decreasing grazing intensity. At the catchment scale, 13 Δ of mature leaves varied from 6.2‰ to 8.5‰ and increased with topsoil silt content. At the regional scale, 13 Δ of mature leaves varied from 5.5‰ to 8.9‰, increasing with growing-season precipitation. At all scales, 13 Δ decreased with increasing leaf nitrogen content (N leaf). N leaf was positively correlated with grazing intensity and leaf position along tillers, but negatively correlated with precipitation. The presence of the correlations across a range of different environmental contexts strongly implicates N leaf as a major driver of 13 Δ in C. squarrosa and, possibly, other C 4 species.