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      Gold Nanoparticle-Aptamer-Based LSPR Sensing of Ochratoxin A at a Widened Detection Range by Double Calibration Curve Method

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          Ochratoxin A (OTA) is a type of mycotoxin generated from the metabolism of Aspergillus and Penicillium, and is extremely toxic to humans, livestock, and poultry. However, traditional assays for the detection of OTA are expensive and complicated. Other than OTA aptamer, OTA itself at high concentration can also adsorb on the surface of gold nanoparticles (AuNPs), and further inhibit AuNPs salt aggregation. We herein report a new OTA assay by applying the localized surface plasmon resonance effect of AuNPs and their aggregates. The result obtained from only one single linear calibration curve is not reliable, and so we developed a “double calibration curve” method to address this issue and widen the OTA detection range. A number of other analytes were also examined, and the structural properties of analytes that bind with the AuNPs were further discussed. We found that various considerations must be taken into account in the detection of these analytes when applying AuNP aggregation-based methods due to their different binding strengths.

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          Most cited references 42

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          Gold nanoparticles in chemical and biological sensing.

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            Localized surface plasmon resonance sensors.

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              Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients


                Author and article information

                Front Chem
                Front Chem
                Front. Chem.
                Frontiers in Chemistry
                Frontiers Media S.A.
                04 April 2018
                : 6
                1State Key Laboratory of Chemical Engineering, School of Chemical Engineering and Technology, Tianjin University , Tianjin, China
                2School of Environmental Science and Engineering, Tianjin University , Tianjin, China
                3Collaborative Innovation Center of Chemical Science and Engineering , Tianjin, China
                Author notes

                Edited by: Huan-Tsung Chang, National Taiwan University, Taiwan

                Reviewed by: Lingxin Chen, Yantai Institute of Coastal Zone Research (CAS), China; Juewen Liu, University of Waterloo, Canada

                *Correspondence: Rongxin Su surx@ 123456tju.edu.cn

                This article was submitted to Analytical Chemistry, a section of the journal Frontiers in Chemistry

                Copyright © 2018 Liu, Huang, Yu, Su, Qi and He.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                Page count
                Figures: 6, Tables: 2, Equations: 0, References: 49, Pages: 9, Words: 5826
                Funded by: National Natural Science Foundation of China 10.13039/501100001809
                Award ID: 51473115
                Award ID: 21776212
                Funded by: Natural Science Foundation of Tianjin City 10.13039/501100006606
                Award ID: 16JCZDJC37900
                Funded by: Ministry of Science and Technology of the People's Republic of China 10.13039/501100002855
                Award ID: 2012YQ090194
                Original Research


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