The effect of a Kenyan strain of sweetpotato leaf curl virus (SPLCV) and its interactions with sweetpotato feathery mottle virus (SPFMV), and sweetpotato chlorotic stunt virus (SPCSV) on root yield was determined. Trials were performed during two seasons using varieties contrasting in their resistance to sweetpotato virus disease, ‘Kakamega’ and ‘Ejumula’, in a randomized complete block design with sixteen treatments replicated three times. The treatments included plants graft inoculated with SPLCV, SPFMV and SPCSV alone and in possible dual or triple combinations. Yield and yield related parameters were evaluated at harvest. Results showed marked differences in the effect of SPLCV infection on the two varieties: ‘Ejumula’, which is susceptible to SPFMV and SPCSV, suffered no significant yield loss from SPLCV infection, whereas ‘Kakamega’, which is more resistant to SPFMV and SPCSV, suffered an average of 47% yield loss, despite only mild symptoms occurring in both varieties. These results highlight the variability in sensitivity to SPLCV between sweetpotato cultivars as well as a lack of correlation of SPLCV related symptoms with susceptibility to the virus. In addition, they underline the lack of correlation between resistance to the RNA viruses SPCSV and SPFMV and DNA virus SPLCV.