34
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      MLST revisited: the gene-by-gene approach to bacterial genomics.

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.

          Related collections

          Author and article information

          Journal
          Nat Rev Microbiol
          Nature reviews. Microbiology
          Springer Science and Business Media LLC
          1740-1534
          1740-1526
          October 2013
          : 11
          : 10
          Affiliations
          [1 ] Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK.
          Article
          nrmicro3093 EMS57777
          10.1038/nrmicro3093
          3980634
          23979428
          8edffe52-18d7-4676-be2f-ffdf96194ad9
          History

          Comments

          Comment on this article