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      Parental transfer of microcystin-LR induced transgenerational effects of developmental neurotoxicity in zebrafish offspring.

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          Abstract

          Microcystin-LR (MCLR) has been reported to cause developmental neurotoxicity in zebrafish, but there are few studies on the mechanisms of MCLR-induced transgenerational effects of developmental neurotoxicity. In this study, zebrafish were exposed to 0, 1, 5, and 25 μg/L MCLR for 60 days. The F1 zebrafish embryos from the above-mentioned parents were collected and incubated in clean water for 120 h for hatching. After examining the parental zebrafish and F1 embryos, MCLR was detected in the gonad of adults and F1 embryos, indicating MCLR could potentially be transferred from parents to offspring. The larvae also showed a serious hypoactivity. The contents of dopamine, dihydroxyphenylacetic acid (DOPAC), serotonin, gamma-aminobutyric acid (GABA) and acetylcholine (ACh) were further detected, but only the first three neurotransmitters showed significant reduction in the 5 and 25 μg/L MCLR parental exposure groups. In addition, the acetylcholinesterase (AChE) activity was remarkably decreased in MCLR parental exposure groups, while the expression levels of manf, bdnf, ache, htr1ab, htr1b, htr2a, htr1aa, htr5a, DAT, TH1 and TH2 genes coincided with the decreased content of neurotransmitters (dopamine, DOPAC and serotonin) and the activity of AChE. Neuronal development related genes, α1-tubulin, syn2a, mbp, gfap, elavl3, shha and gap43 were also measured, but gap43 was the gene only up-regulated. Our results demonstrated MCLR could be transferred to offspring, and subsequently induce developmental neurotoxicity in F1 zebrafish larvae by disturbing the neurotransmitter systems and neuronal development.

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          Author and article information

          Journal
          Environ. Pollut.
          Environmental pollution (Barking, Essex : 1987)
          Elsevier BV
          1873-6424
          0269-7491
          Dec 2017
          : 231
          : Pt 1
          Affiliations
          [1 ] College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, PR China.
          [2 ] Institute of Agricultural Quality Standards & Testing Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, PR China.
          [3 ] College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China.
          [4 ] Department of Biological and Agricultural Engineering, University of California-Davis, Davis, CA 95616, USA.
          [5 ] Chinese Research Academy of Environmental Science, State Key Laboratory of Environmental Criteria and Risk Assessment, State Environmental Protection Key Laboratory for Lake Pollution Control, Beijing 100012, PR China.
          [6 ] College of Fisheries, Huazhong Agricultural University, Wuhan 430070, PR China; Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Wuhan 430070, PR China; Hubei Provincial Engineering Laboratory for Pond Aquaculture, Wuhan 430070, PR China; National Demonstration Center for Experimental Aquaculture Education (Huazhong Agricultural University), Wuhan 430070, PR China. Electronic address: liguangyu@mail.hzau.edu.cn.
          Article
          S0269-7491(17)31734-7
          10.1016/j.envpol.2017.08.038
          28837927
          8ee5220c-c5e4-40eb-a65c-f6b96df4bc34
          History

          Developmental neurotoxicity,MCLR,Neurotransmitter,Transgenerational effects,Zebrafish embryos

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