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      Association of TCTP with Centrosome and Microtubules

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          Abstract

          Translationally Controlled Tumour Protein (TCTP) associates with microtubules (MT), however, the details of this association are unknown. Here we analyze the relationship of TCTP with MTs and centrosomes in Xenopus laevis and mammalian cells using immunofluorescence, tagged TCTP expression and immunoelectron microscopy. We show that TCTP associates both with MTs and centrosomes at spindle poles when detected by species-specific antibodies and by Myc-XlTCTP expression in Xenopus and mammalian cells. However, when the antibodies against XlTCTP were used in mammalian cells, TCTP was detected exclusively in the centrosomes. These results suggest that a distinct pool of TCTP may be specific for, and associate with, the centrosomes. Double labelling for TCTP and γ-tubulin with immuno-gold electron microscopy in Xenopus laevis oogonia shows localization of TCTP at the periphery of the γ-tubulin-containing pericentriolar material (PCM) enveloping the centriole. TCTP localizes in the close vicinity of, but not directly on the MTs in Xenopus ovary suggesting that this association requires unidentified linker proteins. Thus, we show for the first time: (1) the association of TCTP with centrosomes, (2) peripheral localization of TCTP in relation to the centriole and the γ-tubulin-containing PCM within the centrosome, and (3) the indirect association of TCTP with MTs.

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          Most cited references26

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          Cell cycle extracts.

          A W Murray (1991)
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            The translationally controlled tumour protein (TCTP).

            The translationally controlled tumour protein (TCTP) is a highly conserved protein that is widely expressed in all eukaryotic organisms. Based on its sequence, TCTP was listed as a separate protein family in protein databases but the recent elucidation of the solution structure of the fission yeast orthologue places it close to a family of small chaperone proteins. The molecular functions determined so far, Ca(2+)- and microtubule-binding, have been mapped to an alpha-helical region of the molecule. TCTP expression is highly regulated both at the transcriptional and translational level and by a wide range of extracellular signals. TCTP has been implicated in important cellular processes, such as cell growth, cell cycle progression, malignant transformation and in the protection of cells against various stress conditions and apoptosis. In addition, an extracellular, cytokine-like function has been established for TCTP, and the protein has been implicated in various medically relevant processes.
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              Absence of centrioles in the first and second meiotic spindles of mouse oocytes.

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                Author and article information

                Journal
                Biochem Res Int
                Biochem Res Int
                BCRI
                Biochemistry Research International
                Biochemistry Research International
                2090-2247
                2090-2255
                2012
                14 May 2012
                : 2012
                : 541906
                Affiliations
                1Department of Developmental Biology & Invertebrate Morphology, Institute of Zoology, Jagiellonian University, 30-387 Krakow, Poland
                2CNRS, UMR 6290, Institut de Génétique et Développement de Rennes, Cell Cycle Group, 35043 Rennes, France
                3UEB, IFR 140, Faculté de Médecine, Université Rennes 1, 35043 Rennes, France
                4Department of Genetics & Evolution, Institute of Zoology, Jagiellonian University, 30-387 Krakow, Poland
                5Department of Embryology, Institute of Zoology, University of Warsaw, 02-096 Warszawa, Poland
                6Department of Surgery, The Methodist Hospital, Houston, TX 77030, USA
                7Immunobiology Laboratory, The Methodist Hospital Research Institute, Houston, TX 77030, USA
                Author notes

                Academic Editor: R. Mark Ghobrial

                Article
                10.1155/2012/541906
                3359677
                22655198
                8f4f92fe-bad1-4cce-a531-38a08f0a1fa8
                Copyright © 2012 Mariusz K. Jaglarz et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 16 February 2012
                : 6 March 2012
                Categories
                Research Article

                Biochemistry
                Biochemistry

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