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      Low access resistance perforated patch recordings using amphotericin B

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      Journal of Neuroscience Methods

      Elsevier BV

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          Abstract

          We present experimental procedures describing the creation of perforated patches by use of amphotericin B. In 13 different cellular preparations, access resistances below 10 M omega were achieved and with blunt electrode tips, access resistances of 3-4 M omega were possible. In addition to using the techniques to measure whole cell currents, we have used them to measure single channel currents in a new "outside-out patch" preparation and we have utilized them to measure the resting voltage of epithelial monolayers. We conclude that these new approaches can provide a substantial increase in versatility and quality for many kinds of electrophysiological measurements.

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          Author and article information

          Journal
          Journal of Neuroscience Methods
          Journal of Neuroscience Methods
          Elsevier BV
          01650270
          March 1991
          March 1991
          : 37
          : 1
          : 15-26
          Article
          2072734
          © 1991

          https://www.elsevier.com/tdm/userlicense/1.0/

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