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      A Subset of β-Endorphin- or Dynorphin-Containing Neurons in the Medial Basal Hypothalamus Accumulates Estradiol

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          Abstract

          We used the combined steroid autoradiography-immunocytochemical method to determine whether estradiol- or dexamethasone-concentrating cells contain endogenous opioid peptides. Ovariectomized-adrenalectomized female rats were given highly radioactive doses of <sup>3</sup>H-estradiol or <sup>3</sup>H-dexamethasone, then sacrificed to demonstrate nuclear steroid binding. Autoradiograms were prepared, exposed for 2–12 months, photodeveloped, and fixed; immunocytochemistry was carried out on the same sections using antibodies to β-endorphin or dynorphin A (1–17). In the medial basal hypothalamus, many estradiol- and some dexamethasone-concentrating neurons were found intermingled with β-endor-phin or dynorphin-immunoreactive neurons. Of the β-endorphin-immunoreactive neurons in the medial basal hypothalamus, 4% concentrated estradiol in their nuclei. In addition, a subset of β-endorphin-immunoreactive cells in the anterior pituitary concentrated estradiol in their nuclei. Although none of the β-endorphin-immunoreactive neurons in the medial basal hypothalamus concentrated dexamethasone in their nuclei, many of the β-endorphin-immunoreactive cells in the anterior pituitary did. Of the dynorphin-immunoreactive neurons in the medial basal hypothalamus, 10% concentrated estradiol in their nuclei. These data are consistent with the hypothesis of a genomic effect of estradiol on a particular subset of medial basal hypothalamic neurons that produce endogenous opioid peptides.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1985
          1985
          28 March 2008
          : 41
          : 5
          : 417-426
          Affiliations
          aThe Rockefeller University, New York, N.Y.; bEast Carolina University, School of Medicine, Greenville, N.C., USA
          Article
          124212 Neuroendocrinology 1985;41:417–426
          10.1159/000124212
          2865692
          © 1985 S. Karger AG, Basel

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          Page count
          Pages: 10
          Categories
          Original Paper

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