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      Revision of Japanese species of Nipponomyia Alexander, 1924 (Diptera, Pediciidae)

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          Abstract

          Japanese species of the genus Nipponomyia Alexander, 1924 are revised. Two new species, Nipponomyia okinawensis Kolcsár & Kato, sp. nov. and N. yakushimensis Kolcsár & Kato, sp. nov. are described from the Ryukyu Islands. Images of habitus and wings, illustrations of male and female terminalia, and distribution maps are provided for the Japanese species. A key to the world species of Nipponomyia is added. DNA barcodes of three Japanese Nipponomyia are provided, representing the first barcodes from the genus.

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          Clustal W and Clustal X version 2.0.

          M.A. Larkin, G Blackshields, N.P. Brown … (2007)
          The Clustal W and Clustal X multiple sequence alignment programs have been completely rewritten in C++. This will facilitate the further development of the alignment algorithms in the future and has allowed proper porting of the programs to the latest versions of Linux, Macintosh and Windows operating systems. The programs can be run on-line from the EBI web server: http://www.ebi.ac.uk/tools/clustalw2. The source code and executables for Windows, Linux and Macintosh computers are available from the EBI ftp site ftp://ftp.ebi.ac.uk/pub/software/clustalw2/
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            DnaSP v5: a software for comprehensive analysis of DNA polymorphism data.

            P Librado, J Rozas (2009)
            DnaSP is a software package for a comprehensive analysis of DNA polymorphism data. Version 5 implements a number of new features and analytical methods allowing extensive DNA polymorphism analyses on large datasets. Among other features, the newly implemented methods allow for: (i) analyses on multiple data files; (ii) haplotype phasing; (iii) analyses on insertion/deletion polymorphism data; (iv) visualizing sliding window results integrated with available genome annotations in the UCSC browser. Freely available to academic users from: (http://www.ub.edu/dnasp).
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              DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

              M Beier (1966)
              We describe "universal" DNA primers for polymerase chain reaction (PCR) amplification of a 710-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene (COI) from 11 invertebrate phyla: Echinodermata, Mollusca, Annelida, Pogonophora, Arthropoda, Nemertinea, Echiura, Sipuncula, Platyhelminthes, Tardigrada, and Coelenterata, as well as the putative phylum Vestimentifera. Preliminary comparisons revealed that these COI primers generate informative sequences for phylogenetic analyses at the species and higher taxonomic levels.
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                Author and article information

                Contributors
                Levente-Péter Kolcsár:
                ORCID: https://orcid.org/0000-0001-7784-2386
                Journal
                Journal ID (nlm-ta): Zookeys
                Journal ID (iso-abbrev): Zookeys
                Journal ID (publisher-id): 2
                Journal ID (index): urn:lsid:arphahub.com:pub:45048D35-BB1D-5CE8-9668-537E44BD4C7E
                Journal ID (aggregator): urn:lsid:zoobank.org:pub:91BD42D4-90F1-4B45-9350-EEF175B1727A
                Title: ZooKeys
                Publisher: Pensoft Publishers
                ISSN (Print): 1313-2989
                ISSN (Electronic): 1313-2970
                Publication date Collection: 2020
                Publication date (Electronic): 03 December 2020
                Volume: 1000
                Pages: 71-105
                Affiliations
                [1 ] Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577, Japan
                [2 ] Echigo-Matsunoyama Museum of Natural Sciences, ‘Kyororo’, 1712-2 Matsunoyama, Tôkamachi, 942-1411, Japan
                [3 ] Department of Civil and Environmental Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan
                Author notes
                Corresponding author: Levente-Péter Kolcsár ( kolcsar.peter@ 123456gmail.com )

                Academic editor: G. Kvifte

                Author information
                https://orcid.org/0000-0001-7784-2386
                https://orcid.org/0000-0002-7062-595X
                Article
                Publisher ID: 55021
                DOI: 10.3897/zookeys.1000.55021
                PMC ID: 7728730
                SO-VID: 8fcc7750-487e-489b-aed2-23e6736ae8b1
                Copyright © Levente-Péter Kolcsár, Daichi Kato, Maribet Gamboa, Kozo Watanabe
                License:

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                Date received : 02 June 2020
                Date accepted : 15 November 2020
                Categories
                Subject: Research Article
                Subject: Animalia
                Subject: Arthropoda
                Subject: Diptera
                Subject: Hexapoda
                Subject: Insecta
                Subject: Invertebrata
                Subject: Nematocera
                Subject: Pediciidae
                Subject: Tipulomorpha
                Subject: Faunistics & Distribution
                Subject: Identification key
                Subject: Systematics
                Subject: Taxonomy
                Subject: Asia
                Subject: Japan

                ScienceOpen disciplines: Animal science & Zoology
                Keywords: coi,crane flies,distribution,genitalia,new species,ovipositor,taxonomy, tipuloidea
                Data availability:
                ScienceOpen disciplines: Animal science & Zoology
                Keywords: coi, crane flies, distribution, genitalia, new species, ovipositor, taxonomy, tipuloidea

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