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      A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development.

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          Abstract

          Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit multiple attachments to the 5' UTR from the translationally regulated IGF-II leader 3 mRNA but are unable to bind to the 5' UTR from the constitutively translated IGF-II leader 4 mRNA. IMPs contain the unique combination of two RNA recognition motifs and four hnRNP K homology domains and are homologous to the Xenopus Vera and chicken zipcode-binding proteins. IMP localizes to subcytoplasmic domains in a growth-dependent and cell-specific manner and causes a dose-dependent translational repression of IGF-II leader 3 -luciferase mRNA. Mouse IMPs are produced in a burst at embryonic day 12.5 followed by a decline towards birth, and, similar to IGF-II, IMPs are especially expressed in developing epithelia, muscle, and placenta in both mouse and human embryos. The results imply that cytoplasmic 5' UTR-binding proteins control IGF-II biosynthesis during late mammalian development.

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          Author and article information

          Journal
          Mol Cell Biol
          Molecular and cellular biology
          American Society for Microbiology
          0270-7306
          0270-7306
          Feb 1999
          : 19
          : 2
          Affiliations
          [1 ] RNA Regulation Centre, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark.
          Article
          10.1128/MCB.19.2.1262
          116055
          9891060
          8fd3465b-623c-4fd7-a33e-b94ee623ceb6
          History

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