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      Seroprevalencia de babesiosis bovina en la hacienda Vegas de la Clara, Gómez Plata (Antioquia), 2008 Translated title: Seroprevalence of Bovine Babesiosis at the Vegas De La Clara Farm, Gómez Plata (Antioquia), 2008

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          Abstract

          La babesiosis es una enfermedad del ganado bovino transmitida por la garrapata Rhipicephalus (Boophilus) microplus y causada por los parásitos protozoarios Babesia bovis y B. bigemina. Una zona se considera epizootiológicamente estable frente a Babesia spp, cuando el 75% de los bovinos entre las edades de 3 a 9 meses son serorreactivos (IgG) frente a Babesia bovis y Babesia bigemina y no hay evidencia de signos clínicos. El objetivo de esta investigación fue determinar la seroprevalencia de Babesia bovis y Babesia bigemina en el ganado bovino de la hacienda Vegas de la Clara (Universidad de Antioquia, Gómez Plata, Antioquia), por medio de inmunofluorescencia indirecta. Se diseñó un estudio descriptivo prospectivo con análisis de corte transversal. Fue evaluada toda la población bovina de la hacienda Vegas de la Clara (n = 118). Las muestras fueron evaluadas por inmunofluorescencia indirecta para la detección de anticuerpos tipo IgG específicos contra Babesia bovis y B. bigemina. La serorreactividad obtenida en los bovinos evaluados, para al menos una especie de Babesia fue del 89,8%, para Babesia bovis del 83,8%, mientras que para B. bigemina del 61%. Se obtuvo una relación estadísticamente significativa entre la serorreactividad para B. bigemina y la frecuencia del tratamiento garrapaticida. La serorreactividad indica estabilidad enzoótica en el hato para B. bovis, mientras que para B. bigemina se encontró que la frecuencia del tratamiento garrapaticida interrumpe su ciclo de transmisión.

          Translated abstract

          Babesiosis is a cattle disease transmitted by Rhipicephalus (Boophilus) microplus ticks and caused by protozoan parasites Babesia bovis and Babesia bigemina. An area is considered as epizootiologically stable to Babesia spp when 75% of the cattle between the ages of 3 to 9 months are seroreactive (IgG) against Babesia bovis and Babesia bigemina, and there is no clinical evidence. The purpose of this study was to determine the seroprevalence of Babesia bovis and Babesia bigemina in cattle at the Vegas de la Clara Farm (Antioquia University, Gómez Plata, Antioquia) through indirect immunofluorescence. A prospective descriptive study with cross-sectional analysis was designed and the entire bovine population of the Clara Vegas Farm (n = 118) was evaluated. All samples were evaluated through indirect immunofluorescence in order to detect specific IgG antibodies against B. bovis and B. bigemina. The seroreactivity obtained in the evaluated bovines for at least one species of Babesia was of 89.8%, of 83.8% for Babesia bovis and of 61% for B. bigemina. A statistically significant relationship was obtained between the seroreactivity for B. bigemina and the frequency of acaricide treatment. Seroreactivity indicates enzootic stability in the herd for B. bovis, whereas for B. bigemina it was found that the frequency of acaricide treatment interrupts its transmission cycle.

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          Babesia spp. infection in Boophilus microplus engorged females and eggs in Sao Paulo State, Brazil.

          Babesia spp. infections were investigated in Bos taurus x Bos indicus dairy cows and calves and in Boophilus microplus engorged female ticks and eggs. Blood samples and engorged female ticks were collected from 25 cows and 27 calves. Babesia spp. was detected in ticks by microscopic examination of hemolymph of engorged female and by squashes of egg samples. Cattle infection was investigated in blood thin smears and by DNA amplification methods (PCR and nested PCR), using specific primers for Babesia bovis and Babesia bigemina. Merozoites of B. bovis (3 animals) and B. bigemina (12 animals) were detected exclusively in blood smears of calves. DNA amplification methods revealed that the frequency of B. bigemina infection in calves (92.6%) and in cows (84%) and of B. bovis in calves (85.2%) and in cows (100%) did not differ significantly (P > 0.05). Babesia spp. infection was more frequent in female ticks and eggs collected from calves (P < 0.01) than from cows, especially in those which had patent parasitemia. Hatching rates of B. microplus larvae were assessed according to the origin of engorged females, parasitemia of the vertebrate host, frequency and intensity of infection in engorged female tick, and frequency of egg infection. Hatching rate was lower in samples collected from calves (P < 0.01) than from cows, and in those in which Babesia spp. was detected in egg samples (P < 0.01).
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            Normas Científicas, Técnicas y Administrativas para la Investigación en Salud

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              Differential Bos taurus cattle response to Babesia bovis infection.

              Bovine babesiosis is a tick-borne disease caused by Babesia spp. haemoprotozoans. The disease is of great importance at tick enzootic unstable areas and hampers cattle production in several developing countries. The available immunisation alternatives are pre-immunition and attenuated vaccines. Despite being efficient and protective, they are unsafe as they use cattle blood cells as inoculum and may potentially spread other diseases. Another alternative to help in babesiosis control would be the identification of genetically resistant cattle to Babesia bovis infection. The objective of this work was to phenotype cattle based on primary response against B. bovis infection. Two-hundred and forty half-sib Hereford and Aberdeen Angus heifers (120 animals from each breed), 12-18-month-old naïve cattle, originated from a tick-free area in Southern Brazil, were used in the experiment. Animals were monitored following an inoculation with 1x10(7)B. bovis parasitised erythrocytes. Results showed three different phenotypes: 1-'susceptible', animals with babesiosis clinical signs that received treatment to avoid death; 2-'intermediate', animals with clinical signs: parasitaemia, >or=21.5% reduction in packed cell volume (PCV) and increase in body temperature when compared to their pre-challenge physiological parameters, no specific treatment was needed as animals self recovered from the disease, and 3-'resistant', animals without clinical signs that showed B. bovis presence in blood smears, <21.5% PCV reductions, with little or no increase in body temperature and no need for babesiosis treatment. The frequencies of each phenotype were: 45.4, 26.7, and 27.9%, respectively, demonstrating the existence of phenotypic variation for B. bovis in Bos taurus cattle.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rmv
                Revista de Medicina Veterinaria
                Rev. Med. Vet.
                Universidad de La Salle (Bogotá )
                0122-9354
                June 2011
                : 0
                : 21
                : 63-71
                Affiliations
                [1 ] Universidad de Antioquia Colombia
                [2 ] Universidad de Antioquia Colombia
                [3 ] Universidad de Antioquia Colombia
                [4 ] Universidad de Antioquia Colombia
                [5 ] Universidad de Antioquia Colombia
                Article
                S0122-93542011000100005
                8fe1f234-2633-448f-890e-93baaabffbe8

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Colombia

                Self URI (journal page): http://www.scielo.org.co/scielo.php?script=sci_serial&pid=0122-9354&lng=en
                Categories
                VETERINARY SCIENCES

                General veterinary medicine
                babesiosis,indirect immunofluorescence,ticks,acaricides,inmunofluorescencia indirecta,garrapatas,acaricidas

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