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      Electronic separation of biological cells by volume.

      Science (New York, N.Y.)
      Animals, Cell Biology, instrumentation, Cricetinae, Culture Media, Culture Techniques, Electronics, Erythrocytes, Female, Lymphoma, Mice, Ovary, cytology

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          Abstract

          A device capable of separating biological cells (suspended in a conducting medium) according to volume has been developed. Cell volume is measured in a Coulter aperture, and the cells are subsequently isolated in droplets of the medium which are charged according to the sensed volume. The charged droplets then enter an electrostatic field and are deflected into a collection vessel. Mixtures of mouse and human erythrocytes and a large volume component of mouse lymphoma cells were separated successfully. In tests with Chinese hamster ovary cells essentially all cells survived separation and grew at their normal rate.

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          Author and article information

          Journal
          5891056
          10.1126/science.150.3698.910

          Chemistry
          Animals,Cell Biology,instrumentation,Cricetinae,Culture Media,Culture Techniques,Electronics,Erythrocytes,Female,Lymphoma,Mice,Ovary,cytology

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