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Abstract
A simple, rapid and accurate high-performance liquid chromatographic (HPLC) technique
coupled with photodiode array (PDA) detection was developed for the simultaneous determination
of noradrenaline (NA) and dopamine (DA) in Chinese herbal plant extracts from the
different parts of Portulaca oleracea L. The effects of various parameters, such as
sodium dodecyl sulfate (SDS) concentration, pH value and proportion of methanol on
chromatographic behavior of the analytes (NA and DA) were investigated. Separation
of NA and DA was achieved within 10 min by a mobile phase consisting of 70% (v/v)
methanol and 0.02 M potassium dihydrogen phosphate solution, which contained 30 mM
SDS and was adjusted to pH 3.0 with H3PO4. NA and DA showed good linear relationships
in the range of 0.004-6.00 microg and 0.011-8.25 microg respectively. The correlation
coefficients of the calibration curve for the analytes exceeded 0.999. The detection
limits for NA and DA were 0.40 ng and 0.55 ng, at a signal-to-noise ratio of 3, respectively.
Moreover, the optimized HPLC method was employed to analyze three different parts
of Portulaca oleracea L.