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Molecular hybridization of radioactive DNA to the DNA of cytological preparations.

Publication date: 1969-09-30

Journal: Proceedings of the National Academy of Sciences of the United States of America

Keywords: Animals, Autoradiography, DNA, Female, Hybridization, Genetic, Methods, Mice, Ovum, Thymidine, metabolism, Tritium

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Abstract

A method is presented for detecting the cellular location of specific DNA fractions. The technique involves the hybridization of a radioactive test DNA in solution to the stationary DNA of a cytological preparation. Sites of DNA binding are then detected by autoradiography. Experiments with DNA of the toad Xenopus are described.

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PubMed ID:: 5261036

PMC ID:: 223386

DOI:: 10.1073/pnas.64.2.600

ScienceOpen disciplines: Chemistry

Keywords: Animals,Autoradiography,DNA,Female,Hybridization, Genetic,Methods,Mice,Ovum,Thymidine,metabolism,Tritium

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ScienceOpen disciplines: Chemistry

Keywords: Animals, Autoradiography, DNA, Female, Hybridization, Genetic, Methods, Mice, Ovum, Thymidine, metabolism, Tritium

Molecular hybridization of radioactive DNA to the DNA of cytological preparations.

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