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      Identification of essential biofilm proteins in middle ear fluids of otitis media with effusion patients

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          Abstract

          Otitis media with effusion (OME) is a common disease of childhood that is largely asymptomatic. However, middle ear fluid can persist for months and negatively impact a child’s quality of life. Many cases of OME remain chronic and require surgical intervention. As biofilms are known to contribute to the persistence of many diseases, this study examined effusions collected from children with chronic OME for presence of essential biofilm structural components, members of the DNABII family of bacterial DNA-binding proteins. Middle ear effusions were recovered from 38 children with chronic OME at the time of tympanostomy tube insertion. A portion of each specimen was submitted for microbiology culture. The remaining material was assessed by immunoblot to quantitate individual DNABII proteins, integration host factor (IHF) and histone-like protein (HU). Sixty-five percent of effusions (24/37) were culture-positive for bacterial species or yeast, whereas 35% (13/37) were culture-negative. IHF was detected in 95% (36/38) at concentrations from 2– 481 ng/ μl effusion. HU was detected in 95% (36/38) and quantitated from 13– 5,264 ng/ μl effusion ( P ≤ 0.05 compared to IHF). As DNABII proteins are essential structural components of bacterial biofilms, these data lend further support to our understanding that biofilms are present in the vast majority of chronic middle ear effusions, despite negative culture results. The presence and ubiquity of DNABII proteins in OME specimens indicated that these proteins can serve as an important clinical target for our novel DNABII-directed strategy to treat biofilm diseases such as chronic OME.

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          Update on otitis media – prevention and treatment

          Acute otitis media and otitis media with effusion are common childhood disorders, a source of significant morbidity, and a leading cause of antibiotic prescription in primary health care. Although effective treatments are available, some shortcomings remain, and thus better treatments would be welcome. Recent discoveries within the field of otitis media research relating to its etiology and pathogenesis have led to further investigation aimed at developing novel treatments. This article provides a review of the latest evidence relating to the understanding of acute otitis media and otitis media with effusion, current treatment strategies, their limitations, new areas of research, and novel strategies for treatment.
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            Global costs of unaddressed hearing loss and cost-effectiveness of interventions: a WHO report, 2017

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              A bacterial biofilm induced oral osteolytic infection can be successfully treated by immuno‐targeting an extracellular nucleoid associated protein

              Freire MO (2016)
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                Author and article information

                Contributors
                (View ORCID Profile)
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                Journal
                The Laryngoscope
                The Laryngoscope
                Wiley
                0023-852X
                1531-4995
                May 21 2019
                March 2020
                April 25 2019
                March 2020
                : 130
                : 3
                : 806-811
                Affiliations
                [1 ]Center for Microbial PathogenesisNationwide Children's Hospital Columbus Ohio U.S.A.
                [2 ]Department of Pediatric Otolaryngology–Head and Neck SurgeryNationwide Children's Hospital Columbus Ohio U.S.A.
                [3 ]The Ohio State University College of Medicine Columbus Ohio U.S.A.
                Article
                10.1002/lary.28011
                6814449
                31021431
                90f88244-1c35-4188-afa4-c6b75fd23e34
                © 2020

                http://onlinelibrary.wiley.com/termsAndConditions#vor

                http://doi.wiley.com/10.1002/tdm_license_1.1


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