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      Preventive Effect of Natural and Synthetic Antioxidants on Lipid Peroxidation in the Mammalian Eye

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          Abstract

          Lipid peroxidation was initiated with 5 m M ferric iron and aliquots of bovine or porcine retina, purified rod outer segments (ROS) and retinal pigment epithelium (RPE). After 30 min of oxidation, the lipid hydroperoxide content of the tissues increased approximately 4- to 8-fold over control levels with ROS showing the greatest change and RPE the lowest. Eight lipid and aqueous antioxidants from natural or synthetic sources and five flavonoids were evaluated for their ability to inhibit the reaction. Species differences were observed with regard to the fraction under study. Palm oil-derived vitamin E showed significant protection in bovine retina, ROS and RPE. In the porcine eye, the major defense was also afforded by vitamin E, but MCLA and other compounds such as EPC-K1 and U74500A were all quite active at 10<sup>––</sup><sup>5</sup> M concentration. Of the flavonoids tested, partial protection in the bovine retina was found at 10<sup>––5</sup> M levels for epigallocatechin gallate (EGCg), quercetin, diosmetin and pycnogenol. When vitamin E and EGCg or quercetin were combined, the individual effect was enhanced. These results demonstrate the usefulness of an in vitro model system that can rapidly and accurately determine the capacity of antioxidants against lipid peroxidation.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1996
          1996
          11 December 2009
          : 28
          : 3
          : 184-192
          Affiliations
          Department of Clinical Laboratory Science, State University of New York, Buffalo, N.Y., USA
          Article
          267901 Ophthalmic Res 1996;28:184–192
          10.1159/000267901
          8829176
          © 1996 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
          Categories
          Original Paper

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