The Middle East species of Claraeola Aczél (Diptera, Pipunculidae) are revised based on morphological characteristics and sequence data from the mitochondrial COI barcoding gene, using a novel COI mini-barcode protocol. Four new Claraeola species are described: C. bousynterga Motamedinia & Skevington, sp. nov., C. heidiae Motamedinia & Skevington, sp. nov., C. khuzestanensis Motamedinia & Skevington, sp. nov., and C. mantisphalliga Motamedinia & Skevington, sp. nov. Eudorylas thekkadiensis Kapoor, Grewal & Sharma, 1987 is transferred to Claraeola, C. thekkadiensis ( comb. nov.). Diagnoses, illustrations, an identification key, and a distributional map are given for the Middle East species.