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      Correction to: Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non-human primates from the Brazilian Atlantic Forest

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          Abstract

          Correction to: Malar J (2018) 17:338 10.1186/s12936-018-2485-0 Following publication of the original article [1], it was flagged that one of the authors (Anielle de Pina Costa) is missing an affiliation in the article. Please note the author Anielle de Pina Costa also has this affiliation: Centro Universitário Serra dos Órgãos (UNIFESO), Teresópolis/RJ, 25964-004, Brazil. The author apologizes for this error.

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          Frozen blood clots can be used for the diagnosis of distinct Plasmodium species in man and non-human primates from the Brazilian Atlantic Forest

          Background Zoonotic infections with epidemic potential, as non-human primate malaria and yellow fever (YF), can overlap geographically. Optimizing a small blood sample for diagnosis and surveillance is of great importance. Blood are routinely collected for YF diagnosis and blood clots usually discarded after serum obtention. Aiming to take sample advantage, the sensitivity of a PCR using extracted DNA from long-term frozen clots from human and non-human primates for detection of Plasmodium spp. in low parasitaemia conditions was assayed. Results Malaria diagnosis with DNA extracted from blood clots generated results in agreement with samples obtained with whole blood, including mixed Plasmodium vivax/simium and Plasmodium malariae/brasilianum infections. Conclusion Blood clots from human and non-human primates may be an important and low cost source of DNA for malaria surveillance in the Atlantic Forest.
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            Author and article information

            Contributors
            lourenco@ioc.fiocruz.br
            mffcruz28@gmail.com
            Journal
            Malar J
            Malar. J
            Malaria Journal
            BioMed Central (London )
            1475-2875
            14 May 2019
            14 May 2019
            2019
            : 18
            : 168
            Affiliations
            [1 ]ISNI 0000 0001 0723 0931, GRID grid.418068.3, Laboratório de Mosquitos Transmissores de Hematozoários, , Instituto Oswaldo Cruz, Fiocruz, ; Rio de Janeiro, Brazil
            [2 ]Instituto Federal do Norte de Minas Gerais Campus Salinas, Minas Gerais, Brazil
            [3 ]ISNI 0000 0001 0723 0931, GRID grid.418068.3, Laboratório de Pesquisa em Malária, , Instituto Oswaldo Cruz, Fiocruz, ; Rio de Janeiro, Brazil
            [4 ]ISNI 0000 0001 0723 0931, GRID grid.418068.3, Centro de Pesquisa, Diagnóstico e Treinamento em Malária, , Instituto Oswaldo Cruz, Fiocruz, ; Rio de Janeiro, Brazil
            [5 ]ISNI 0000 0001 0723 0931, GRID grid.418068.3, Laboratório de Doenças Febris Agudas, , Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, ; Rio de Janeiro, Brazil
            [6 ]Divisão de Vigilância Ambiental em Saúde, Secretaria de Saúde do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul Brazil
            [7 ]ISNI 0000 0001 2238 5157, GRID grid.7632.0, Faculdade de Agronomia e Veterinárias, , Universidade de Brasília, ; Brasília, Brazil
            [8 ]GRID grid.442239.a, Centro Universitário Serra dos Órgãos (UNIFESO), ; Teresópolis, RJ 25964-004 Brazil
            Author information
            http://orcid.org/0000-0003-3522-3792
            Article
            2804
            10.1186/s12936-019-2804-0
            6515590
            31088475
            91877865-b0ab-4d0f-8797-cc3988cb107c
            © The Author(s) 2019

            Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License ( http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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            © The Author(s) 2019

            Infectious disease & Microbiology
            Infectious disease & Microbiology

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