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      Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.

      The Journal of Biological Chemistry
      Amino Acid Sequence, Animals, Chromatography, High Pressure Liquid, Electrochemistry, Lactoglobulins, analysis, Membranes, Methods, Myoglobin, Polyvinyls, Rosaniline Dyes

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          Abstract

          Small amounts (7-250 pmol) of myoglobin, beta-lactoglobulin, and other proteins and peptides can be spotted or electroblotted onto polyvinylidene difluoride (PVDF) membranes, stained with Coomassie Blue, and sequenced directly. The membranes are not chemically activated or pretreated with Polybrene before usage. The average repetitive yields and initial coupling of proteins spotted or blotted into PVDF membranes ranged between 84-98% and 30-108% respectively, and were comparable with the yields measured for proteins spotted onto Polybrene-coated glass fiber discs. The results suggest that PVDF membranes are superior supports for sequence analysis of picomole quantities of proteins purified by gel electrophoresis.

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          Author and article information

          Journal
          3611052
          10.1016/S0021-9258(18)61070-1

          Chemistry
          Amino Acid Sequence,Animals,Chromatography, High Pressure Liquid,Electrochemistry,Lactoglobulins,analysis,Membranes,Methods,Myoglobin,Polyvinyls,Rosaniline Dyes

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