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      The last battle of Anne of Brittany: Solving mass grave through an interdisciplinary approach (paleopathology, biological anthropology, history, multiple isotopes and radiocarbon dating)

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          Abstract

          Mass graves are usually key historical markers with strong incentive for archeological investigations. The identification of individuals buried in mass graves has long benefitted from traditional historical, archaeological, anthropological and paleopathological techniques. The addition of novel methods including genetic, genomic and isotopic geochemistry have renewed interest in solving unidentified mass graves. In this study, we demonstrate that the combined use of these techniques allows the identification of the individuals found in two Breton historical mass graves, where one method alone would not have revealed the importance of this discovery. The skeletons likely belong to soldiers from the two enemy armies who fought during a major event of Breton history: the siege of Rennes in 1491, which ended by the wedding of the Duchess of Brittany with the King of France and signaled the end of the independence of the region. Our study highlights the value of interdisciplinary approaches with a particular emphasis on increasingly accurate isotopic markers. The development of the sulfur isoscape and testing of the triple isotope geographic assignment are detailed in a companion paper [ 13].

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          Collagen turnover in the adult femoral mid-shaft: modeled from anthropogenic radiocarbon tracer measurements.

          We have measured the (14)C content of human femoral mid-shaft collagen to determine the dynamics of adult collagen turnover, using the sudden doubling and subsequent slow relaxation of global atmospheric (14)C content due to nuclear bomb testing in the 1960s and 1970s as a tracer. (14)C measurements were made on bone collagen from 67 individuals of both sexes who died in Australia in 1990-1993, spanning a range of ages at death from 40 to 97, and these measurements were compared with values predicted by an age-dependent turnover model. We found that the dataset could constrain models of collagen turnover, with the following outcomes: 1) Collagen turnover rate of females decreases, on average, from 4%/yr to 3%/yr from 20 to 80 years. Male collagen turnover rates average 1.5-3%/yr over the same period. 2) For both sexes the collagen turnover rate during adolescent growth is much higher (5-15%/yr at age 10-15 years), with males having a significantly higher turnover rate than have females, by up to a factor of 2. 3) Much of the variation in residual bomb (14)C in a person's bone can be attributed to individual variation in turnover rate, but of no more than about 30% of the average values for adults. 4) Human femoral bone collagen isotopically reflects an individual's diet over a much longer period of time than 10 years, including a substantial portion of collagen synthesised during adolescence.
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            A method for visual determination of sex, using the human hip bone.

            J Brůzek (2002)
            A new visual method for the determination of sex using the human hip bone (os coxae) is proposed, based on a revision of several previous approaches which scored isolated characters of this bone. The efficacy of the methodology is tested on a sample of 402 adults of known sex and age of French and Portuguese origins. With the simultaneous use of five characters of the hip bone, it is possible to provide a correct sexual diagnosis in 95% of all cases, with an error of 2% and an inability to identify sex in only 3%. The advantage of this new method is a reduction in observer subjectivity, since the evaluation procedure cannot involve any anticipation of the result. In addition, this method of sex determination increases the probability of a correct diagnosis with isolated fragments of the hip bone, provided that a combination of elements of one character is found to be typically male or female. Copyright 2002 Wiley-Liss, Inc.
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              Age Variation of Formation Stages for Ten Permanent Teeth

                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: Formal analysisRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: SupervisionRole: Writing – original draftRole: Writing – review & editing
                Role: InvestigationRole: Project administrationRole: ValidationRole: Writing – review & editing
                Role: InvestigationRole: SupervisionRole: ValidationRole: Writing – review & editing
                Role: Formal analysisRole: InvestigationRole: MethodologyRole: ValidationRole: Writing – review & editing
                Role: Formal analysisRole: InvestigationRole: Methodology
                Role: Formal analysisRole: Methodology
                Role: Formal analysisRole: InvestigationRole: Methodology
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: Supervision
                Role: Formal analysisRole: InvestigationRole: MethodologyRole: Writing – review & editing
                Role: InvestigationRole: MethodologyRole: Validation
                Role: Formal analysis
                Role: Formal analysisRole: InvestigationRole: Methodology
                Role: Formal analysisRole: InvestigationRole: Methodology
                Role: Formal analysisRole: InvestigationRole: SupervisionRole: Validation
                Role: SupervisionRole: ValidationRole: Writing – original draft
                Role: ConceptualizationRole: Formal analysisRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                5 May 2021
                2021
                : 16
                : 5
                : e0248086
                Affiliations
                [1 ] INRAP (Institut National de Recherches Archéologiques Préventives), Cesson-Sévigné, France
                [2 ] CNRS, CAGT, UMR 5288, Université Paul Sabatier, 31000, Toulouse, France
                [3 ] Department of Earth and Environmental Sciences, University of Ottawa, Ottawa, Canada
                [4 ] Department of Biology, University of Ottawa, Ottawa, Canada
                [5 ] Tempora, Université Rennes 2, Rennes, France
                [6 ] CNRS, CReAAH, UMR 6566, Rennes, France
                [7 ] Max Planck Institute for Evolutionary Anthropology, Department of Human Evolution, Leipzig, Germany
                [8 ] I2MC, Université de Toulouse, INSERM U1048, 31432, Toulouse, France
                [9 ] Department of Forensic Medicine, Centre Hospitalier Universitaire de Toulouse, Université de Toulouse, Toulouse, France
                [10 ] Faculté de Médecine Rangueil, CMEAB, Université de Toulouse, Toulouse, France
                [11 ] CNRS, GET UMR 5563, Observatoire Midi Pyrénées, Toulouse, France
                Museo delle Civiltà, ITALY
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                https://orcid.org/0000-0001-9417-9053
                https://orcid.org/0000-0003-2820-5035
                Article
                PONE-D-21-05103
                10.1371/journal.pone.0248086
                8099129
                33951047
                91d3dbca-bbc1-4ef2-9fd2-03509ce57757
                © 2021 Colleter et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 18 February 2021
                : 6 April 2021
                Page count
                Figures: 7, Tables: 0, Pages: 25
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/501100000781, European Research Council;
                Award ID: 803676
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100001659, Deutsche Forschungsgemeinschaft;
                Award ID: 378496604
                Award Recipient :
                Funded by: National Sciences and Engineering Research Council (NSERC)
                Award ID: RGPIN-2019-05709
                Award Recipient :
                Funded by: Max Planck Society
                KJ: European Research Council "ARCHEIS 803676" https://erc.europa.eu/ MT: Deutsche Forschungsgemeinschaft "PALEODIET 378496604" https://www.dfg.de/ CB: National Sciences and Engineering Research Council (NSERC) "Discovery Grant RGPIN-2019-05709 " https://www.nserc-crsng.gc.ca/ Cost of isotope analyses was funded by the Max Planck Society.
                Categories
                Research Article
                Biology and Life Sciences
                Anatomy
                Digestive System
                Teeth
                Medicine and Health Sciences
                Anatomy
                Digestive System
                Teeth
                Biology and Life Sciences
                Anatomy
                Head
                Jaw
                Teeth
                Medicine and Health Sciences
                Anatomy
                Head
                Jaw
                Teeth
                Research and Analysis Methods
                Chemical Characterization
                Isotope Analysis
                Physical Sciences
                Physics
                Particle Physics
                Composite Particles
                Atoms
                Isotopes
                People and places
                Geographical locations
                Europe
                European Union
                France
                Biology and Life Sciences
                Biochemistry
                Proteins
                Collagens
                Biology and Life Sciences
                Anatomy
                Musculoskeletal System
                Skeleton
                Medicine and Health Sciences
                Anatomy
                Musculoskeletal System
                Skeleton
                Physical Sciences
                Chemistry
                Chemical Elements
                Sulfur
                Biology and Life Sciences
                Nutrition
                Diet
                Medicine and Health Sciences
                Nutrition
                Diet
                Custom metadata
                All relevant data are within the paper and its Supporting Information files.

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