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      Changes in microvascular reactivity after cardiopulmonary bypass in patients with poorly controlled versus controlled diabetes.


      pharmacology, Vasodilator Agents, drug effects, Vasoconstriction, analysis, analogs & derivatives, Tyrosine, Substance P, genetics, biosynthesis, Proto-Oncogene Proteins c-akt, Protein Processing, Post-Translational, Phosphorylation, Nitroprusside, blood supply, Muscle, Skeletal, Middle Aged, physiology, Microcirculation, Male, physiopathology, etiology, Inflammation, therapeutic use, Hypoglycemic Agents, Humans, Hemoglobin A, Glycosylated, Gene Expression Regulation, Female, Enzyme Induction, metabolism, Endothelium, Vascular, Disease Susceptibility, drug therapy, complications, blood, Diabetes Mellitus, Type 2, Cyclic AMP-Dependent Protein Kinases, Coronary Artery Bypass, adverse effects, Cardiopulmonary Bypass, Arterioles, Aged, Adenosine Diphosphate, 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid

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          We investigated the effects of cardiopulmonary bypass (CPB) on peripheral arteriolar reactivity and associated signaling pathways in poorly controlled (UDM), controlled (CDM), and case-matched nondiabetic (ND) patients undergoing coronary artery bypass grafting (CABG). Skeletal muscle arterioles were harvested before and after CPB from the UDM patients (hemoglobin A1c [HbA1c]=9.0 ± 0.3), the CDM patients (HbA1c=6.3 ± 0.15), and the ND patients (HbA1c=5.2 ± 0.1) undergoing CABG surgery (n=10/group). In vitro relaxation responses of precontracted arterioles to endothelium-dependent vasodilators adenosine 5'-diphosphate (ADP) and substance P and the endothelium-independent vasodilator sodium nitroprusside (SNP) were examined. The baseline responses to ADP, substance P, and SNP of arterioles from the UDM patients were decreased as compared with microvessels from the ND or CDM patients (P<0.05). The post-CPB relaxation responses to ADP and substance P were significantly decreased in all 3 groups compared with pre-CPB responses (P<0.05). However, these decreases were more pronounced in the UDM group (P<0.05). The post-CPB response to SNP was significantly decreased only in the UDM group, not in the other 2 groups compared with pre-CPB. The expression of protein kinase C (PKC)-α, PKC-β, protein oxidation, and nitrotyrosine in the skeletal muscle were significantly increased in the UDM group as compared with those of ND or CDM groups (P<0.05). Poorly controlled diabetes results in impaired arteriolar function before and after CPB. These alterations are associated with the increased expression/activation of PKC-α and PKC-β and enhanced oxidative and nitrosative stress.

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