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      Suppression of abnormal morphology and extracytoplasmic function sigma activity in Bacillus subtilis ugtP mutant cells by expression of heterologous glucolipid synthases from Acholeplasma laidlawii.

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          Abstract

          Glucolipids in Bacillus subtilis are synthesized by UgtP processively transferring glucose from UDP-glucose to diacylglycerol. Here we conclude that the abnormal morphology of a ugtP mutant is caused by lack of glucolipids, since the same morphology arises after abolition of glucolipid production by disruption of pgcA and gtaB, which are involved in UDP-glucose synthesis. Conversely, expression of a monoglucosyldiacylglycerol (MGlcDG) produced by 1,2-diacylglycerol 3-glucosyltransferase from Acholeplasma laidlawii (alMGS) almost completely suppressed the ugtP disruptant phenotype. Activation of extracytoplasmic function (ECF) sigmas (SigM, SigV, and SigX) in the ugtP mutant was decreased by alMGS expression, and was suppressed to low levels by MgSO4 addition. When alMGS and alDGS (A. laidlawii 1,2-diacylglycerol-3-glucose (1-2)-glucosyltransferase producing diglucosyldiacylglycerol (DGlcDG)) were simultaneously expressed, SigX activation was repressed to wild type level. These observations suggest that MGlcDG molecules are required for maintenance of B. subtilis cell shape and regulation of ECF sigmas, and DGlcDG regulates SigX activity.

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          Author and article information

          Journal
          Biosci Biotechnol Biochem
          Bioscience, biotechnology, and biochemistry
          Informa UK Limited
          1347-6947
          0916-8451
          Dec 2016
          : 80
          : 12
          Affiliations
          [1 ] a Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering , Saitama University , Saitama , Japan.
          Article
          10.1080/09168451.2016.1217147
          27684739
          9264ac8a-6629-4df5-ba47-550a64ab1c14
          History

          Bacillus subtilis,ugtP,ECF sigma,glucolipid,cell membrane integrity

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