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      Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification.

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          Abstract

          The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates.

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          Author and article information

          Journal
          Appl. Environ. Microbiol.
          Applied and environmental microbiology
          American Society for Microbiology
          1098-5336
          0099-2240
          Aug 2011
          : 77
          : 15
          Affiliations
          [1 ] Akihiro Ohnishi, Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, 1-1 Sakuragaoka 1-chome, Setagaya-ku, Tokyo 156-8502, Japan. a1ohnish@nodai.ac.jp
          Article
          AEM.00359-11
          10.1128/AEM.00359-11
          3147432
          21705538
          929f3a82-f32a-412b-907c-dd43e517483e
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